POT1-INTERACTING PROTEIN PIP1

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。人类端粒长度由基于TRF1与双链端粒DNA结合的负反馈环控制。TRF1复合体招募POT1，一种单链端粒DNA结合蛋白，是顺式抑制端粒酶所必需的。通过质谱分析，我们鉴定了一种新的端粒蛋白，命名为POT1相互作用蛋白1(PIP1)。PIP1结合POT1和TRF1相互作用因子TIN2，并能将POT1与TRF1复合体连接。用shRNAs降低PIP1或POT1的水平导致端粒延长，表明PIP1通过招募POT1参与端粒长度控制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Human telomere length is controlled by a negative feedback loop based on the binding of TRF1 to doublestranded telomeric DNA. The TRF1 complex recruits POT1, a single-stranded telomeric DNA-binding protein necessary for cis-inhibition of telomerase. By mass spectrometry, we have identified a new telomeric protein, which we have named POT1-interacting protein 1 (PIP1). PIP1 bound both POT1 and the TRF1-interacting factor TIN2 and could tether POT1 to the TRF1 complex. Reduction of PIP1 or POT1 levels with shRNAs led to telomere elongation, indicating that PIP1 contributes to telomere length control through recruitment of POT1.