Animal Studies

核心C是动物核心。核心将为临床前项目1、2建立提供技术服务 皮下和全身肿瘤模型，评估放射性标记药物的摄取，确定治疗 放射性标记抗体结合物和半抗原的有效性以及与单峰和多峰相关的毒性鉴定 治疗方案。它还将收集血液进行毒性评估，并收集组织进行组织学分析 .By Core E.它将使用2名F/T技术人员在其AAALAC认证的设施中每年管理2160-2560只鼠标。 具体地说，它将负责以下任务：(A)开发新的未经治疗的和 免疫抑制小鼠，(B)将培养的肿瘤细胞静脉或皮下注射到SCID小鼠或裸鼠体内 小鼠，(C)给动物注射适当的治疗剂[例如，双特异性抗体，放射性标记的半抗原， 放射性抗体、裸露抗体和化疗药物]，(D)监测存活、体重变化和发病情况 全身性肿瘤模型后腿瘫痪的研究及三维卡尺测量皮下肿瘤大小 测量，(E)进行生物分布研究，(F)为组织学提供肿瘤和组织的固定样本 通过免疫组织学和显微放射自显影研究来监测生存能力和抗原的表达， (G)采集血液样本，进行白细胞和血小板计数，并测定#年的心肌肌钙蛋白 项目4，(H)将所有数据(生物分布、治疗、WBC/Plt、体重)输入Excel电子表格，以及(1) 建立免疫核素扫描研究。

Core C is the animal core. The core will provide technical service to preclinical projects 1, and 2 establishing subcutaneous and systemic tumor models, assessing uptake of radiolabeled agents, determining therapeutic efficacy of radiolabeled antibody conjugates and haptens, and identifying toxicity associated with single and multimodal treatment protocols. It will also collect blood for toxicity assessments and tissue for the histological analysis .by Core E. It will use 2 F/T technicians to manage 2160-2560 mice per year in its AAALAC-accredited facility. Specifically it will be responsible for the following tasks: (a) developing new tumor models in untreated and immunosuppressed mice, (b) injecting cultured tumor cells intravenously or subcutaneously in SCID mice or nude mice, (c) dosing animals with appropriate therapeutic agents [e.g., bispecific antibodies, radiolabeled haptens, radioantibodies, naked antibodies, and chemotherapeutics], (d) monitoring survival, body weight changes and onset of hind-leg paralysis in systemic tumor models and measuring tumor size of subcutaneous tumors by 3D caliper measurements, (e) performing biodistribution studies, (f) providing fixed samples of tumors and tissues for histology to monitor viability and to monitor expression of antigens by immunohistology and for microautoradiography studies, (g) collecting blood samples for white blood cell and platelet enumeration and for measurement of cardiac troponin in project 4, (h) entering all data (biodistribution, therapy, WBC/Plt, body weight) into Excel spreadsheets, and (i) setting up immunoscintigraphy studies.