Identification of new plant defense signaling pathways

就像哺乳动物的几种细菌病原体一样，许多植物病原细菌注入蛋白质 进入宿主细胞的细胞质。植物对细菌侵袭的识别导致通过以下途径介导的防御反应 至少有三条防御信号通路。为了测试是否存在额外的植物防御信号通路， 我将确定参与这些途径的基因。我将使用全基因组微阵列分析 鉴定拟南芥对细菌病原菌假单胞菌诱导的基因 丁香科，但与涉及水杨酸的典型防御信号通路无关地被触发， 茉莉酸和乙烯。我将对其中192个基因进行突变分析，以确定这些基因 它们可能参与植物防御所需的新的疾病信号通路 细菌病原体。我将研究这些途径在植物抵御病原体方面的作用 多样化的殖民战略。此外，我将详细说明这些通路是如何触发的，它们是如何发挥作用的， 以及它们是如何组织起来的。因为这些研究将确定所需的新的植物信号通路 对于抗病而言，它们对于研究植物对微生物侵袭的免疫反应至关重要。

As is the case with several bacterial pathogens of mammals, many plant pathogenic bacteria inject proteins into the host cell cytoplasm. Plant recognition of bacterial attack results in a defense response mediated by at least three defense signaling pathways. To test whether additional plant defense signaling pathways exist, I will identify genes that participate in these pathways. I will use whole genome microarray analysis to identify Arabidopsis thaliana genes that are induced in response to the bacterial pathogen Pseudomonas syringae but are triggered independently of the canonical defense signaling pathways involving salicylic acid, jasmonic acid and ethylene. I will perform mutational analysis of 192 of these genes to identify those genes that are likely to participate in novel disease signaling pathways that are required for plant defense against bacterial pathogens. I will study the role of these pathways in plant protection against pathogens with diverse colonization strategies. Further, I will detail how these pathways are triggered, how they function, and how they are organized. As these studies will identify novel plant signaling pathways that are required for disease resistance, they are crucial to the study of the plant immune response to microbial attack.