FOCAL ADHESION KINASE 2

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。FAK 2是一种参与将信号从细胞外部传递到细胞内部的分子，允许细胞对外部刺激做出反应。由于许多功能障碍可能会出现这种信号通路的错误调节，进一步了解所涉及的分子动力学和相互作用将在未来的治疗方面产生严重的影响。我们计划收集FAK 2粘着斑靶向和FERM结构域的完整数据集，以及这些结构域与各自底物结合的共晶。这些晶体将在不使用任何重原子衍生物的情况下制备。然而，可以使用硒代甲硫氨酸衍生物，并且需要MAD数据收集。所有带入工厂的物品/样品将与我们一起返回并妥善处理。此外，我们的小组目前正在努力解决FGF受体激酶结构域在其各种磷酸化状态下的结构。这样做，结合结合实验，将使我们能够完全理解FGFR激酶的激活/磷酸化顺序和磷酸化顺序背后的结构原因。FGFR与许多疾病和癌症有关，关于其激活模式的见解具有极大的科学意义。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. FAK2 is a molecule involved in relaying signals from the outside to the inside of the cell, allowing the cell to respond to external stimuli. As many disfunctions can arise throught the misregulation of such signaling pathways, a further understanding of the molecular dynamics and interactions involved will have serious implications in terms of future therapeutic treatments. We plan to collect full data sets of the FAK2 focal adhesion-targeting and FERM domains as well as co-crystals of these domains bound to their respective substrates. These crystals will be prepared without the usage of any heavy atom derivatives. However, seleno-methionine derivatives may be used and would require MAD data collection. All items/samples brought into the facility will return with us and be properly disposed of. Furthermore, our group is currnetly working on solving the structures of the FGF receptor kinase domain in its various phosphorylated states. Doing so, in combination with binding experiments, will allow us to completely understand the order of activation/phosphorylation of the FGFR kinase and structural reasons behind the order of phosphorylation. FGFR is involved in numerous disorders and cancers, and such insight as to its mode of activation is of extreme scientific interest.