Optical Coherence Microscopy for Endoscopic Diagnostics

这个提议的假设是光学相干显微镜(OCM)，OCT和OCT的组合 共焦显微镜，可以实现细胞分辨率的组织形态内窥镜成像，从而 极大地提高了这两种技术中任何一种在早期阶段进行内窥镜检查的能力 肿瘤。一种内窥镜“光学活组织检查”技术，允许实时、活体成像 细胞水平上的癌变将允许传统切除活检的图像指导， 潜在地减少了采样误差并提高了灵敏度。具体目标是：1)发展光学 相干显微镜(OCM)技术用于未染色组织的细胞成像。2)调查OCM 通过体外临床成像研究检测人体组织中早期肿瘤细胞的变化。3) 基于微型机电系统的OCM成像小型化柔性内窥镜的研制 (MEMS)扫描技术。4)通过活体动物成像验证柔性OCM内窥镜 实验。这些研究结合起来试图展示内窥镜检查的基本新进展。 早期肿瘤的检测。

The hypothesis of this proposal is that optical coherence microscopy (OCM), a combination of OCT with confocal microscopy, can enable cellular resolution endoscopic imaging of tissue morphology, thereby dramatically advancing the capabilities of either technique for endoscopic detection of early stage neoplasms. An endoscopic "optical biopsy" technique that allows for real time, in vivo imaging of pre- cancerous changes at the cellular level would allow image guidance of traditional excisional biopsy, potentially reducing sampling error and improving sensitivity. The specific aims are: 1) Develop optical coherence microscopy (OCM) technology for cellular imaging of unstained tissues. 2) Investigate OCM for detecting early neoplastic cellular alterations in human tissues through ex vivo clinical imaging studies. 3) Develop miniaturized flexible endoscopes for OCM imaging based on micro-electro-mechanical systems (MEMS) scanning technology. 4) Validate flexible OCM endoscopes through in vivo animal imaging experiments. These studies combined seek to demonstrate fundamental new advances for endoscopic detection of early stage neoplasms.