Identifying Regulation of Cell Cycle Gene Networks in E*

这项研究将主要在印度加尔各答的博斯研究所与阿努拉达合作完成 罗伊亚作为美国国立卫生研究院拨款R01AI 53724的延伸。 人类病原体溶组织内阿米巴可引起阿米巴痢疾和肝脏 脓肿。人体肠道感染后，寄生虫很快就会繁殖繁殖， 这可能会导致结肠病。我们对识别调节细胞的分子因子很感兴趣 在这种原生寄生虫的扩散，以努力制定新的战略，以防止阿米巴病。的目标是 父母的建议是通过基于微阵列的转录来鉴定新的阿米巴毒力因子 侧写。FIRCA补编是与Anuradha Lohia教授共同撰写的，他一直在研究这项规定 细胞周期进程的组织溶解内阿米巴。 Anuradha在印度的实验室的结果表明，在溶组织埃希氏菌中，基因组复制可能 发生在没有细胞分裂的情况下，单个核在增殖过程中可能包含几个基因组内容 阶段(Gangopadhyay等人，1997a；Das和Lohia，2002年)。因此，与大多数真核生物不同，细胞周期 似乎不受已知的检查点监视机制控制。如果是这样的话 因此，重要的是要确定全球范围内调节细胞周期进程的基因在组织裂解杆菌中的分布。 在这项提案中，我们计划使用dsRNA干扰(Kaur和Lohia，2004)来下调细胞周期 同源物-Eh CDKs和Cyclins，然后使用微阵列进行转录图谱分析以识别 由CDKs和Cyclins控制的基因群。这项工作具有重要的意义 鉴定溶组织乳杆菌细胞周期控制的新方面。3.此外，这个项目是一个理想的项目 以技术转让为主要目标的协作环境(微阵列数据的使用和分析) 到印度的罗伊亚实验室。作为一种后基因组工具，阵列功能强大但高度专业化，因此 Lohia的团队对这一应用程序的“动手”使用是这项工作的重要组成部分。

This research will be done primarily at the Bose Institute in Calcutta, India in collaboration with Anuradha Lohia as an extension of NIH grant R01AI 53724. The human pathogen Entamoeba histolytica is responsible for causing amoebic dysentery and liver abscesses. Infection of the human gut is quickly followed by proliferation and multiplication of the parasite, which may then lead to colonic disease. We are interested in identifying molecular factors regulating cell proliferation in this protist parasite, in an effort to devise new strategies to prevent amoebiasis. The goal of the parent proposal is to identify novel amoebic virulence factors by microarray based transcriptional profiling. The FIRCA supplement is written with Prof. Anuradha Lohia who has been studying the regulation of cell cycle progression Entamoeba histolytica. Results from Anuradha's laboratory in India have shown that in E. histolytica genome reduplication may occur without cell division and single nuclei may contain several genome contents during the proliferative phase (Gangopadhyay et al, 1997a; Das and Lohia, 2002). Therefore, unlike most eukaryotes the cell cycle of E. histolytica does not appear to be controlled by known checkpoint surveillance mechanisms. It would be important therefore to identify a global profile of genes that regulate cell cycle progression in E. histolytica. In this proposal we plan to use dsRNA interference (Kaur and Lohia, 2004) to down-regulate cell cycle homologues- Eh Cdks and cyclins, followed by transcriptional profiling using microarrays in order to identify groups of genes controlled by CDKs and cyclins in E. histolytica. This work has important implications for identifying novel aspects of cell cycle control in E. histolytica. .Additionally, this project is an ideal collaborative environment in which a main goal is technology transfer (use and anlaysis of microarray data) to the Lohia lab in India. Arrays as a post-genomic tools are powerful yet highly specialized, thus the "hands-on" use of this application by Lohia's group is an important component of this work.