Mouse Core

小鼠核心将负责繁殖和提供项目所需的所有小鼠， 方案项目。这三个项目对这一核心资源的预期使用情况如下：项目1（40%），项目2（40%），项目3（40%），项目4（40%），项目5（40%），项目6（40%），项目7（40%），项目8（ 2（30%）、项目3（30%）。该小鼠核心将成为Wakeland小鼠群（WMC）的组成部分， 其是含有> 1500个笼的SPF群体（~5000个每日普查）。这一核心有两个具体目标。的 第一个具体目标是维持PPG研究者所需的所有各种小鼠品系， 生产并提供小鼠用于所有实验。这将需要维护40多个 独特的老鼠品系核心将负责维护育种记录，验证 所有B6同源株的基因型，并根据需要产生小鼠的实验队列， 项目1、2和3的人员进行实验。第二个具体目标将是生产和基因型 所有项目都会产生新的菌株。这将包括培育所有转基因创始人 菌株并将所选转基因创始者渗入到特定基因型上。核心也是 负责饲养和运送小鼠给生物医学研究界的其他研究人员 请求使用我们收藏的老鼠

The mouse core will be responsible for breeding and providing all mice needed by the Projects within the Program project. The anticipated usage of this core by these three Projects will be: Project 1 (40%), Project 2 (30%), Project 3 (30%). This mouse core will be a component of the Wakeland mouse colony (WMC), which is an SPF colony containing >1500 cages (~5000 daily census). This core has two specific aims. The first specific aim will be to maintain all of the various mouse strains required by PPG investigators and to produce and provide mice for use in all experiments. This will require the maintenance of more than 40 unique strains of mice. The core will be responsible for maintaining breeding records, validating the genotypes of all the B6-congenic strains, and producing experimental cohorts of mice as needed for experiments by personnel in Projects 1, 2, and 3. The second specific aim will be to produce and genotype the new strains that will be produced by all of the projects. This will include breeding all transgene founder strains and introgressing the selected transgene founders onto specific genotypes. The core is also responsible for breeding and shipping mice to other investigators in the biomedical research community requesting access to the mice in our collection.