Core--ANIMAL, GENOTYPING, CELL & TISSUE (AGCT)

动物、基因分型、细胞和组织（AGCT）核心将为动物育种、促进、标准化和实施动物基因分型以及组织样本的收集、管理和分析提供技术和人员支持。AGCT核心机构将为所有需要协助的研究者提供以下服务：（1）动物护理、育种和免疫接种，（2）组织和/或体液收集，（3）标记辅助回交过程沿着项目ny Finnegan、Zhang和Mikecz的所有基因分型（“speed congenic”）方法，（4）基本细胞培养方法，（5）用于评估关节炎发展和进展的标准化测试，和（6）处理用于组织学和组织样品评价的样品。AGCT核心旨在通过提供技术、仪器和人员支持来帮助每个项目，这些支持（i）通常由几个项目使用，或（ii）偶尔由调查人员使用，但需要特殊的技术或安全法规，或（iii）特殊设备或技术经验。此外，AGCT Core还提供基本的统计和必要的生物信息学分析。AGCT核心设施，根据这一应用的预期需求，将包括四个单位。动物护理核心单位将设计并进行基因型控制育种，根据研究者的需求对动物进行免疫接种，并以PI独立（盲法）方式评估关节炎的进展。因此，该单位将为所有项目提供服务，生产和测试可供个别项目使用的动物。基因分型核心单元为所有需要基因分型的项目提供服务，沿着回交过程。这包括用于基因组DNA分离的样品收集、记录保存和标记动物，以及具有以下特征的动物的基因分型： 菌株特异性多态性标记。由于大量动物和多个同源/亚同源系，Glant项目的标记辅助育种过程和选择具有适当（所需）重组的动物不属于该核心。另一方面，同一个人（该核心的负责人和Glant项目的共同研究者）将控制所有动物育种和基因分型实验。细胞和组织测试核心股将通过收集动物组织和人类软骨、制备免疫抗原以及标准化测试的组织和血清样本来支持所有研究人员。该单位将负责样本储存、记录保存和采集样本的数据管理，并对组织学标本进行评价/评分。该核心单元将帮助研究者收集细胞或组织进行专门分析（例如外周血、脾细胞或建立原代细胞培养物），进行细胞的组织学和/或基因分型测试，并储存组织样本进行进一步分析。生物信息学核心单位将标准化和进行统计分析，并支持研究者进行数据管理。

The Animal, Genotyping, Cell & Tissue (AGCT) Core will provide technical and personnel support for breeding animals, facilitating, standardizing, and implementing animal genotyping and the collection, management and analysis of tissue samples. The AGCT Core Facility will serve all investigators who will be needing assistance with (1) animal care, breeding and immunization, (2) collection of tissue and/or body fluid, (3) all genotyping for Projects ny Finnegan, Zhang, and Mikecz along the marker-assisted backcrossing ("speed congenic") processes, (4) basic cell culture methods, (5) standardized testing for assessment of the development and progression of arthritis, and (6) processing samples for histology and evaluation of tissue samples. The AGCT Core intends to aid each project by providing technical, instrumental and personnel support which are (i) commonly used by several projects or (ii) occasionally used by investigators but require special technical or safety regulations or (iii) special equipment or technical experience. In addition, the AGCT Core offers basic statistical and necessary bioinformatic analysis. The AGCT Core facility, based on the expected demands of this application, will consist of four units. The Animal Care Core Unit will design and perform genotype-controlled breeding, immunize animals according to the needs of the investigators and assess the progression of arthritis in PI-independent (blind) manner. Thus, this Unit will serve all projects generating and testing animals ready for use by individual projects. The Genotyping Core Unit serves all projects needing genotyping along the backcrossing processes. This includes sample collection for genomic DNA isolation, record keeping and labeling animals, and genotyping of animals with strain-specific polymorphic markers. Due to the large number of animals and multiple congenic/sub-congenic lines, the marker-assisted breeding process of Project by Glant and selection of animals with appropriate (required) recombinations does not belong to this core. On the other hand, the same person (director of this core and co-investigator of Project by Glant ) will control all animal breeding and genotyping experiments. The Cell and Tissue Testing Core Unit will support all investigators by collecting animal tissues and human cartilage, preparing antigens for immunization, and tissue and serum samples for standardized testing. This unit will be responsible for sample storage, record keeping and data management of collected samples, and evaluate/score histological specimens. This core unit will help investigators collect cells or tissues for specialized analysis (e.g. peripheral blood, spleen cells, or establish primary cell cultures), perform histology and/or genotyping tests of cells, and store tissue samples for further analyses. The BiostatisticallBioinformatics Core Unit will standardize and perform statistical analysis and support investigators in data management.