Liquid Array Protein Assay Platform

描述（由申请人提供）：该项目的目的是将基于编码可分选颗粒（ESP）技术的新型“液体阵列”平台的应用扩展到蛋白质测定领域。ESP是光学编码的微制造颗粒，可以使用磁力进行操纵。我们将调整我们的基于ESP的液体阵列系统，该系统已成功应用于DNA领域，以在第一阶段纳入基于抗体的蛋白质检测分析，我们将在第二阶段扩大蛋白质检测的覆盖范围。I期将包括6重细胞因子检测分析，而II期旨在适应1000重或更多的测定。在蛋白质领域，ESP平台在成本、通量、可扩展性、易用性和灵活性方面优于现有的基于珠的液体阵列和固定阵列平台。ESP技术具有广泛的适用性，并且应该是诊断、预后和表征各种疾病状态（包括癌症、心脏病、关节炎和炎性疾病、肾病、肝病、过敏反应和传染病（例如HIV））的有价值的工具。 公共卫生相关性：ESP技术应用于蛋白质检测领域将为许多不同类型的检测提供广阔的平台。ESP平台本质上是多功能的，并且有潜力用于基础生物医学研究以及高通量发现和临床诊断。磁性控制颗粒的能力（以显著不同于磁珠的方式）是显著的优点。该技术应可用于诊断和预后各种疾病状态，包括癌症、心脏病、关节炎和炎性疾病、肾病和传染病（例如HIV）。

DESCRIPTION (provided by applicant): The aim of this project is to extend application of a novel `liquid array' platform, based on Encoded Sortable Particle (ESP) technology, into the protein assay realm. ESPs are optically-encoded microfabricated particles that can be manipulated using magnetic force. We will adapt our ESP-based liquid array system, which has already been successfully applied in the DNA space, to incorporate antibody-based protein detection assays in Phase I, and we will extend the scope of protein assay coverage in Phase II. Phase I will consist of a 6-plex cytokine detection analysis, while Phase II is intended to accommodate assays of 1000-plex or more. In the protein space the ESP platform has significant advantages in cost, throughput, scalability, ease of use, and flexibility over existing bead-based liquid array and fixed array platforms. ESP technology has broad applicability and should be a valuable tool in the diagnosis, prognosis, and characterization of a variety of disease states, including cancer, heart disease, arthritis and inflammatory diseases, kidney disease, liver disease, allergic responses, and infectious diseases (e.g. HIV). PUBLIC HEALTH RELEVANCE: ESP technology as applied to the protein assay space will provide a broad platform for many different kinds of assays. The ESP platform is intrinsically versatile, and has the potential to be used in basic biomedical research, as well as high-throughput discovery and clinical diagnostics. The ability to control the particles magnetically (in a manner significantly different from magnetic beads) is a significant advantage. This technology should be useful in the diagnosis and prognosis of a variety of disease states, including cancer, heart disease, arthritis and inflammatory diseases, kidney disease, and infectious diseases (e.g. HIV).