BIOLOGICAL TESTING CORE

以备使用。生物检测核心设施已全面投入运行 目前。我们已经获得了必要的过滤设备，蝌蚪检测设备和 膜制剂设备。研究股的共享设施将免费提供使用， 包括离心机(低速、中速和高速)、冷藏室、伽马和闪烁计数器 还有冰柜。 2.f.关于生物检测核心设施的引用文献。 [1]Covey，DF，Nathan，D，Kalkbrenner，M，Nilsson，KR，Hu，Y，Zorumski，Cf和Ever，AS， 孕酮诱导的γ-氨基丁酸(A)受体的对映选择性调节 麻醉。药理学与实验治疗学杂志。293：1009-1016,2000。 [2]Franks，NP和Lieb，WR，挥发性全身麻醉剂在分子和细胞上的选择性作用 级别。《英国麻醉杂志》。1993年，71：65-76。 [3]GEE、KW和Lan、NC、Gamma-氨基丁酸A受体复合体在大鼠额叶皮质和 脊髓对类固醇调节表现出不同的反应。分子药理学。40：995- 999,1991年。 [4]Halsey，MJ，Wardley-Smith，B and Wood，S，Alphaxone/Alphadolone和 蝌蚪体内的甲己酮：全身麻醉的不同分子部位的证据。英国人 药理学杂志。89：299-305,1986。 [5]Majewska，MD，Harison，NL，Schwartz，RD，Barker，JL和Paul，SM，类固醇激素代谢物 是类巴比妥酸盐的GABA受体调节剂。科学。232：1004-1007,1986。 [6]Paradiso，K，Sabey，K，Ever，As，Zorumski，CF，Covey，DF和Steinbach，JH，类固醇抑制 大鼠神经元型烟碱型A4B2受体在HEK 293细胞中的表达分子药理学。 58：341-351,2000。 [7]斯里尼瓦桑，S，萨普，DW，托宾，AJ和奥尔森，RW，双相调制GABA受体结合 类固醇的作用说明了功能上的关联。神经化学研究。24：1363-1372,1999。 [8]Tonner，PH，Poppers，DM和Miller，KW，异丙酚的全麻效力及其 对静水压力的依赖。麻醉学。77：926-931,1992。 [9]Turner，DM，Ransom，RW，Yang，JS和Olsen，RW，类固醇麻醉药和自然产生的类似物 调节γ-氨基丁酸受体复合体的活性

for use. The Biological Testing Core Facility is fully operational at present. We have acquired the necessary filtration apparatus, testing equipment for tadpoles and equipment for membrane preparations. The Research Unit shared facilities will be freely available for use, including centrifuges (low-, intermediate- and high-speed), cold room, gamma and scintillation counters and freezers. 2.F. LITERATURE CITED FOR THE BIOLOGICAL TESTING CORE FACILITY. [1] Covey, DF, Nathan, D, Kalkbrenner, M, Nilsson, KR, Hu, Y, Zorumski, CF and Evers, AS, Enantioselectivity of pregnanolone-induced gamma-aminobutyric acid(A) receptor modulation and anesthesia. Journal of Pharmacology and Experimental Therapeutics. 293:1009-1016, 2000. [2] Franks, NP and Lieb, WR, Selective actions of volatile general anaesthetics at molecular andcellular levels. British Journal of Anaesthesia. 71:65-76, 1993. [3] Gee, KW and Lan, NC, Gamma-aminobutyric acidA receptor complexes in rat frontal cortex and spinal cord show differential responses to steroid modulation. Molecular Pharmacology. 40:995- 999, 1991. [4] Halsey, MJ, Wardley-Smith, B and Wood, S, Pressure reversal of alphaxalone/alphadolone and methohexitone in tadpoles: evidence for different molecular sites for general anaesthesia. British Journal of Pharmacology. 89:299-305, 1986. [5] Majewska, MD, Harrison, NL, Schwartz, RD, Barker, JL and Paul, SM, Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor. Science. 232:1004-1007, 1986. [6] Paradiso, K, Sabey, K, Evers, AS, Zorumski, CF, Covey, DF and Steinbach, JH, Steroid inhibition of rat neuronal nicotinic a4 B2 receptors expressed in HEK 293 cells. Molecular Pharmacology. 58:341-351,2000. [7] Srinivasan, S, Sapp, DW, Tobin, AJ and Olsen, RW, Biphasic modulation of GABA receptor binding by steroids suggests functional correlates. Neurochemical Research. 24:1363-1372, 1999. [8] Tonner, PH, Poppers, DM and Miller, KW, The general anesthetic potency of propofol and its dependence on hydrostatic pressure. Anesthesiology. 77:926-931, 1992. [9] Turner, DM, Ransom, RW, Yang, JS and Olsen, RW, Steroid anesthetics and naturally occurring analogs modulate the gamma-aminobutyric acid receptor complex at a