iCys Laser Scanning Cytometer for UC Davis Core

描述（由申请人提供）：CompuCyte iCys激光扫描细胞仪是一种多功能研究成像显微镜，结合了流式细胞术、自动荧光显微镜和图像分析的元素。第一代Compucyte激光扫描细胞仪（LSC）由加州大学戴维斯分校医学院于1997年购买，用于光学生物学核心设施内的共享使用。在过去的10年里，LSC一直在加州大学戴维斯分校积极使用，导致超过15个出版物和12个新的赠款使用该技术。目前，来自6名研究人员的9笔NIH赠款依赖于一个可运作的LSC。从2007年年中开始，CompuCyte将不再支持旧型号的LSC，因此购买新的iCys LSC对完成这些资助项目至关重要。此外，新的iCys LSC不仅具有更新的软件和计算机系统，而且采用倒置显微镜格式，允许新的应用包括活细胞成像，96孔板高通量测定和多载玻片处理。为iCys LSC上的扩展服务合同和共聚焦模块提供机构支持，以允许在荧光原位杂交、亚核蛋白动力学、细胞内包涵体和原代神经元细胞器研究中对小的核内斑点进行高通量定量分析。在加州大学戴维斯分校校园或周边地区没有同等的设备可以满足这些NIH资助项目的定量成像需求。具有共聚焦模块的iCys LSC将对自闭症和精神发育迟滞等常见儿科疾病以及痴呆、共济失调、失明、癌症和糖尿病等常见年龄相关疾病的跨学科生物医学研究产生重大影响。这是加州大学戴维斯分校卫生系统和NIH的共同目标。在具有强大支持记录的核心设施中放置共享iCys LSC成像系统是支持加州大学戴维斯分校NIH资助研究人员的一种具有成本效益的方式。

DESCRIPTION (provided by applicant): The CompuCyte iCys Laser Scanning Cytometer is a versatile research imaging microscope that combines elements of flow cytometry, automated fluorescent microscopy, and image analysis. A first generation Compucyte Laser Scanning Cytometer (LSC) was purchased by the UC Davis School of Medicine in 1997 for shared use within the Optical Biology Core facility. In the last 10 years, the LSC has been actively used at UC Davis, resulting in more than 15 publications and 12 new grants using the technology. Currently, 9 funded NIH grants from 6 investigators are dependent on an operational LSC. Starting in mid-2007, the older model LSC will no longer be supported by CompuCyte, so the purchase of a new iCys LSC is essential to the completion of these funded projects. In addition, the new iCys LSC not only has an updated software and computer system, but is in an inverted microscope format, allowing new applications to include live cell imaging, 96-well plate high throughput assays, and multi-slide processing. Institutional support is provided for an extended service contract and a confocal module on the iCys LSC to allow the high-throughput quantitative analyses of small intra-nuclear spots in fluorescence in situ hybridization, sub-nuclear protein dynamics, intra-cellular inclusions, and organelle studies in primary neurons. There is no equivalent equipment on the UC Davis campus or surrounding area that would serve the quantitative imaging needs of these NIH-funded programs. The iCys LSC with confocal module will strongly impact inter-disciplinary biomedical research on the common pediatric disorders of autism and mental retardation, as well as the common age-related disorders of dementia, ataxia, blindness, cancer, and diabetes. These are common goals of the UC Davis Health System and the NIH. The placement of a shared iCys LSC imaging system in a core facility with a strong track record of support is a cost effective way of supporting NIH funded researchers at UC Davis.