Liver Tissue Cell Line

雷林博士和斯特罗姆博士将继续领导肝组织核心中心，该中心为 大量PAAR调查人员。此核心的目的是维护一个受控的、共享的 用于药物代谢酶研究的特征良好的肝组织(例如，CYP3A、UGT1A1、UGT2B7)，AS 以及在肝组织中表达的相关调控基因(如PXR、CAR)。肝组织核心位于物理位置 在SJ的报警、监控的-70℃冷冻机中。此核心的可用性具有以下优势：(1)质量控制， 来源、最初器官采集、加工、冷冻和储存的标准化和文件记录；(2)质量 控制亚细胞分离程序以分离微粒体、胞浆、RNA和DNA；(3)最大 不同研究人员项目的组织使用效率，例如细胞质、微生物体和基因组DNA，可能都是 从同一肝组织分离；和(4)肝脏特征的集中和保密记录：例如药物 不同细胞色素P450酶的代谢表型、剪接变异体转录本的表达、药物摄取史 组织病理学评估和生殖系基因多态。

Dr. Relling, along with Dr. Strom, will continue to direct the Liver Tissue Core, which provides critical services for a large number of PAAR investigators. The purpose of this Core is to maintain a controlled, shared source of well-characterized liver tissue for research regarding drug-metabolizing enzymes (e.g. CYP3A, UGT1A1, UGT2B7), as well as related regulatory genes (e.g. PXR, CAR) expressed in hepatic tissue. The Liver Tissue Core is physically located in alarmed, monitored -70¿C freezers at SJ. Availability of this Core has the following advantages: (1) quality control, standardization, and documentation of source, initial organ collection, processing, freezing, and storage; (2) quality control over subcellular fractionation procedures for isolation of microsomes, cytosol, RNA, and DNA; (3) maximal efficiency of use of tissue, e.g. cytosol, microsomes, and genomic DNA for different researchers' projects may all be isolated from the same liver tissue; and (4) centralized and confidential record-keeping for liver characteristics: e.g. drug metabolism phenotype for various cytochrome P450 enzymes, expression of splice variant transcripts, drug intake history histopathologic assessment, and germline genotypes for polymorphisms.