CORE--NEUROSCIENCE CORE

核心B，神经科学核心，旨在促进在每个国家进行的研究 本计划的项目项目赠款(PPG)，通过为项目1-4常见的一些“核心”活动提供援助和支持。因为所有4个项目都需要抗体 作为拟议研究的基本工具，一项主要活动将是产生、表征抗体探针，并向这项PPGo的所有调查人员提供抗体探针。第二项核心活动对项目1-4的成功也至关重要，它提供了所有试剂，用于精确测量几种夹心ELISA中的不同淀粉样β蛋白(ABeta)片段，包括Abeta1-40和Abeta1-42以及ABetax-40和ABetax-42。由于项目2-4将在大多数拟议的研究中使用各种单一转基因(TG)小鼠和过度表达淀粉样前体蛋白(APP)和其他几种蛋白质的双基因小鼠，神经科学核心将对各种APP转基因小鼠进行初步杂交、基因分型和一些初步特征。一旦确定了特征，繁殖配对将被 提供给项目2、3和4的调查员，以供进一步扩展。但是，核心B将 继续保持所有转基因小鼠的小队列，以便它们可以补充不同的 为成功完成PPG中提议的实验所需的项目。Core B还将继续为PPG的研究人员提供其他试剂，包括啮齿类神经元的原代培养、人NT2N神经元以及各种病毒试剂。总而言之，通过为本PPG中的所有项目提供许多基本的科学需求，神经科学核心将在成功实现本PPG的研究目标方面发挥至关重要的作用。

Core B, the Neuroscience Core, is designed to facilitate the research conducted in each of the Projects of this Program Project Grant (PPG) by providing assistance and support for a number of 'core' activities that are common to Projects 1-4. Since all 4 Projects will require antibodies as essential tools for the proposed studies, a major activity will be the generation, characterization and provision of antibody probes to all investigators in this PPGo A second core activity which is also essential to the success of Projects 1-4 is the provision of all reagents for the precise measurements of the different amyloid Beta (ABeta) fragments including Abeta1-40 and Abeta1-42 as well as ABetax-40 and ABetax-42 in several sandwich ELISAs. Since Projects 2-4 will use a variety of single transgenic (Tg) and bigenic mice overexpressing amyloid precursor protein (APP) and several other proteins for most of the proposed studies, the Neuroscience Core will perform the initial cross-breeding, genotyping and some initial characterization of the various APP Tg mice. Once characterized, breeding pairs will be provided to the investigators of Projects 2, 3 and 4 for further expansion. However, Core B will continue to maintain small cohorts of all the Tg mice such that they can supplement the different Projects as needed for the successful completion of the experiments proposed in the PPG. Core B will also continue to provide other reagents including primary cultures of rodent neurons, human NT2N neurons as well as various viral reagents for investigators of the PPG. In summary, by providing for many essential scientific needs of all of the Projects in this PPG, the Neuroscience Core will play a vital role in contributing to the successful accomplishment of the research goals of this PPG.