CORE--Proteomics Facility

描述（由申请人提供）：蛋白质组学设施核心旨在开发、采用和维护用于蛋白质组学研究的最先进的专业知识和设备，并提供针对异生物质代谢酶及其转运蛋白的独特抗体，供中心成员使用。 该核心提供蛋白质组学服务，包括2-D凝胶，荧光2-D差异凝胶电泳，用于同时定量处理和未处理的组织或细胞之间数百种蛋白质的差异，并通过蛋白酶消化点的质谱法鉴定这些蛋白质。此外，该设施核心提供了使用独特和不断增长的100多种单克隆和多克隆抗体库的机会和经验，这些抗体对不同的异生物质代谢酶及其转运蛋白具有特异性。

Description (provided by applicant): The Proteomics Facility Core is designed to develop, adopt, and maintain state-of-the-art expertise and equipment for studies in proteomics, and to provide unique antibodies against xenobiotic metabolizing enzymes and their transporters for use by Center members. The core provides proteomic services including 2-D gels, fluorescent 2-D-difference gel electrophoresis for simultaneous quantification of the differences between treated and untreated tissues or cells in hundreds of individual proteins, and identification of these proteins by mass spectrometry of protease digested spots. In addition, the facility core provides access to and experience in the use of a unique and growing library of over 100 monoclonal and polyclonal antibodies specific for different xenobiotic metabolizing enzymes and their transporters.