Facility Core D--Cell Imaging & Analysis

组织和分离细胞的成像是确定生化和分子的关键工具 与组织、细胞和亚细胞细胞器的表型和功能变化有关的机制 因接触外源生物而引起的。成像、生物化学和分子生物学的最新进展 生物学，已经导致成像技术的升级和在生物医学科学中的使用 过去的几年里。荧光探针、绿色荧光蛋白(GFP)、共聚焦和 双光子光学和光电探测器使单分子可视化成为可能， 监测活细胞中的分子相互作用，并增强成像复杂组织的能力。这个 开发最先进的成像技术所需的复杂性、时间和成本使其 个人调查人员不可能独立获得这些重要的工具和方法 生物医学研究。因此，需要一个具有专门知识的有组织的专家小组。 在成像技术中获取仪器，掌握应用，并协助个人 调查人员利用这项技术回答具体的研究问题。 细胞成像和分析设施的长期目标是提供访问最先进的 在流式细胞术和细胞分类、组织病理学和显微镜方面的仪器和专业知识。我们的 目标是开发基于荧光的技术，以满足中心当前和未来的需求 调查人员。我们的基本原理是，使用最先进的荧光技术将使中心 研究人员在细胞水平上确定异种生物作用的具体机制。有动力的人 该设施的互动式工作人员在成像和分析的互补领域提供专业知识。Dr。 Kerkvliet和Oughton女士拥有40多年的流式细胞术和 卓越的研究效率记录。格林伍德博士和弗莱利女士最近建立了 共焦显微镜和宽视场显微镜系统，发表由以下技术发展而来的研究 光漂白后的荧光恢复和荧光共振能量转移显微镜。Dr。 Lohr是一名注册兽医病理学家，与Center进行了重要的协作互动 最近的出版物证明了调查人员的存在，费舍尔女士在 组织学、图像分析和电子显微镜。最后，我们与分子公司的合作 探测器/卵黄素，提供了获得试剂和专业知识的途径，使我们特别能够 完成我们的目标。

Imaging of tissues and isolated cells is a critical tool for determining the biochemical and molecular mechanisms involved in phenotypic and functional changes in tissues, cells, and subcellular organelles induced by exposure to xenobiotics. Recent advances in imaging, biological chemistry, and molecular biology, have led to an escalation in imaging technologies and use in the biomedical sciences over the last several years. Development of fluorescent probes, green fluorescent protein (GFP), confocal and two-photon optics, and photoelectronic detectors, have allowed the visualization of single molecules, monitoring of molecular interactions in live cells, and enhanced ability to image complex tissues. The complexity, time, and cost required for developing state-of-the-art imaging technologies makes it unfeasible for individual investigators to independently obtain these important tools and approaches for biomedical research. Therefore, the need exists for an organized group of specialists with the expertise in imaging technologies to acquire the instrumentation, master the applications, and assist individual investigators in using the technology to answer specific research questions. The long-term goal of the Cell Imaging and Analysis Facility is to provide access to state-of-the-art instrumentation and expertise in flow cytometry and cell sorting, histopathology, and microscopy. Our objective is to develop fluorescence-based technologies to meet the current and future needs of Center investigators. Our rationale is that access to state-of-the-art fluorescence technologies will allow Center investigators to determine specific mechanisms of xenobiotic action at the cellular level. The motivated and interactive staff of the facility provides expertise in complementary areas of imaging and analysis. Dr. Kerkvliet and Ms. Oughton have over 40 years combined experience in flow cytometry with an outstanding record of research productivity. Dr. Greenwood and Ms. Fraley have recently setup the confocal and widefield microscopy systems, publishing studies resulting from the development of fluorescence recovery after photobleaching and fluorescence resonance energy transfer microscopy. Dr. Lohr is a certified Veterinary Pathologist with important collaborative interactions with Center investigators demonstrated by recent publications and Ms. Fischer has over 20 years of experience in histology, image analysis, and electron microscopy. Lastly, our collaboration with Molecular Probes/lnvitrogen, providing access to reagents and expertise, makes us especially well-positioned to accomplish our objective.