PROJECT 6

项目六：逆转录酶复合体(目标三) 项目负责人：Nicolas Sluis-Cremer 将病毒单链(+)RNA基因组反转录成双链DNA是一种 这是人类免疫缺陷病毒(艾滋病毒)生命周期中必不可少的一步。虽然重组纯化病毒 逆转录酶(RT)可以完成DNA的聚合、链转移、链置换等过程。 核糖核酸酶H的体外活性，在新感染的细胞中，逆转录反应是通过以下方式完成的 存在于逆转录复合体(RTC)中的RT分子，该RTC包括其他病毒和 细胞因子207‘208。在这方面，除RT外，RTCs还被证明含有病毒RNA、tRNALysS (作为反转录的引子)、新合成的病毒DNA、基质、核衣壳 蛋白酶、VPR和整合酶207。几个宿主因素，如HMGI、自整合障碍因子和镜头 上皮源性生长因子/p75也可能与RTC207相关。然而，令人惊讶的是，没有主持人 已经确定了与RT特异相互作用的细胞因子。

Project VI: Reverse Transcriptase Complex (Aim III) Project Leader: Nicolas Sluis-Cremer Reverse transcription of the viral single-stranded (+) RNA genome into double-stranded DNA is an essential step in the human immunodeficiency virus (HIV) life-cycle. Although recombinant purified viral reverse transcriptase (RT) can accomplish the DNA polymerization, strand-transfer, strand displacement, and ribonuclease H activities in vitro, in newly infected cells, the reverse transcription reaction is accomplished by RT molecules that are present within a reverse transcription complex (RTC) that includes other viral and cellular factors207'208. In this regard, RTCs have been shown to contain, in addition to RT, viral RNA, tRNALysS (which serves as a primer for reverse transcription), newly synthesized viral DNA, matrix, nucleocapsid, protease, Vpr, and integrase207. Several host factors such as HMGI, barrier to autointegration factor, and lens epithelium-derived growth factor/p75 may also be associated with the RTC207. Surprisingly, however, no host cell factors have been identified that specifically interact with RT.