Molecular and Genomic Imaging Center

产品说明：（申请人提供）我们在此提议建立分子和基因组成像中心（MGIC），以满足整个生物医学界对灵活、具有成本效益、高分辨率技术的需求，从而在基因组和转录组水平上识别和表征生物系统中的变异。我们计划通过开发聚合酶菌落（polony）技术来帮助满足这一需求。Polonies代表了一种高度并行的核酸分析方法，它是现实的，近在咫尺的，模块化的和通用的。MGIC的主要使命是将技术开发人员的各种贡献有效地整合到一个强大的平台中，该平台可以顺利地传播给具有专业临床和生物学兴趣的各种用户。其主要目的是：（1）高度平行的荧光原位测序。(2)转录组的单分子谱，特别是神经分化和哺乳动物选择性剪接。(3)直接分子单体型分析和长距离序列连接。(4)表征来自单细胞的DNA和RNA，特别是表征哺乳动物干细胞中的不对称细胞分裂。(5)核酸分析中处理组合和空间模式的计算算法和系统建模。(6)ELSI的一个组成部分侧重于将技术转化为临床应用的问题和对匿名概念的挑战。

DESCRIPTION: (provided by applicant) We propose here the Molecular and Genomic Imaging Center (MGIC) in response to a biomedical-community-wide need for flexible, cost-effective, high-resolution technology to identify and characterize variation in biological systems at the level of genomes and transcriptomes. We plan to help meet this need by developing the polymerase colony, or polony, technology. Polonies represent a highly parallel method of nucleic acid analysis that is realistic, close-at-hand, modular, and versatile. The primary mission of the MGIC is to efficiently integrate a diverse set of contributions from technology developers into a robust platform that can be smoothly disseminated to a variety of users with specialized clinical and biological interests. These are the main aims: (1) Highly parallel fluorescent in situ sequencing. (2) Single molecule profiling of the transcriptome, in particular of neural differentiation and of mammalian alternative splicing. (3) Direct molecular haplotyping and long-range sequence connectivity. (4) Characterization of DNA & RNA from single cells, in particular characterizing asymmetric cell division in mammalian stem cells. (5) Computational algorithms and systems modeling addressing combinatorial and spatial patterns in nucleic acid analysis. (6) An ELSI component focuses on issues of translation of technology to clinical applications and challenges to the concepts of anonymity.