OCT Imaging Technology for Developmental Cardiology Research

描述（由申请人提供）：心脏病仍然是美国发病率和死亡率的主要原因，2001年造成超过1，400，000人死亡。技术上的限制阻碍了我们改善一组重要的心脏疾病--先天性心脏病--的影响的能力。在这个五年的提案中描述的多学科项目将开发和验证新的调查和诊断技术的基础上新兴的成像技术，光学相干断层扫描（OCT）。OCT能够在体内和真实的时间内以微米级分辨率同时成像小生物样品中的结构和功能，并且潜在地能够测量机电事件。一个多学科的方法是必要的，有针对性地开发这些新的工具，以提高我们对先天性心脏病的机制的理解。具体目标1：开发和演示OCT成像技术用于发育心脏病学研究应用，并传播给合作者进行验证和基线研究。具体目标2：开发和演示图像分析和可视化方法，并传播给合作者进行验证和基线研究。具体目标3：将OCT技术应用于鸡胚正常和异常心脏形态发生的研究。纵向分析。具体目标4：验证并应用OCT技术筛选和分析基因改变小鼠系的心脏缺陷。圆锥动脉干和室壁异常的分析。本项目的目标是开发和验证一套新的工具，将联合收割机结构和生理信息，在迄今为止无法实现的分辨率尺度更好地了解正常和异常的心脏形态发生的机制。这些工具的效用，使实验，以前是不可能的，将建立正常和异常的心脏形态发生的鸡和小鼠模型的基线研究。

DESCRIPTION (provided by applicant): Heart disease remains the leading cause of morbidity and mortality in the United States, responsible for over 1,400,000 deaths in 2001. Limitations in technology have hampered our ability to ameliorate the impact of an important group of cardiac disorders, congenital heart disease. The multi-disciplinary project described in this five-year proposal will develop and validate new investigative and diagnostic technologies based on the emerging imaging technique, optical coherence tomography (OCT). OCT is capable of simultaneous imaging of structure and function in small biological samples with micrometer-scale resolution in vivo and in real time, and is potentially capable of measuring electromechanical events. A multi-disciplinary approach is necessary to target the development of these new tools for the purpose of improving our understanding of the mechanisms of congenital heart disease. Specific Aim 1: Develop and demonstrate OCT imaging technologies for developmental cardiology research applications and disseminate to collaborators for validation and baseline studies. Specific Aim 2: Develop and demonstrate image analysis and visualization methods and disseminate to collaborators for validation and baseline studies. Specific Aim 3: Validate and apply OCT technologies to investigations of normal and abnormal cardiac morphogenesis in the chick embryo. Longitudinal analysis. Specific Aim 4: Validate and apply OCT technologies to screen and analyze cardiac defects in genetically altered mouse lines. Analysis of conotruncal and ventricular wall abnormalities. The goal of this project is to develop and validate a novel set of tools that will combine structural and physiologic information at heretofore unachievable resolution scales for better insight into the mechanisms of normal and abnormal cardiac morphogenesis. The utility of these tools to enable experiments that were previously not possible will be established by baseline studies of chick and mouse models of normal and abnormal cardiac morphogenesis.