Identification and characterization of novel PI3-kinase signal transducing elements in platelets

血小板在凝血和血管修复中起着重要作用。在血管损伤时，血小板迅速粘附在损伤部位，在那里它们发生剧烈的形状变化，并在损伤部位扩散。这些过程的激活和调控依赖于一个复杂的信号转导过程网络，涉及多个受体和途径的整合。磷脂酰肌醇3-激酶（pi3 -激酶）及其脂质产物PtdIns(3,4,5)P3和PtdIns(3,4)P2已被证明在调节血小板反应中发挥作用。然而，关于pi3激酶及其产物如何调节血小板反应的确切机制知之甚少。我们的研究旨在通过鉴定和表征与pi3激酶脂质产物结合的新型血小板蛋白，并确定这些蛋白在血小板pi3激酶依赖性反应中发挥的作用，从而更深入地了解血小板中pi3激酶信号转导的分子机制。

Platelets play an important role in blood clotting and blood vessel repair. Upon vessel injury, platelets rapidly adhere to the site of damage where they undergo dramatic shape change to spread over the site of injury. Activation and regulation of these processes relies on a complex network of signal transduction processes, involving the integration of multiple receptors and pathways. One pathway demonstrated to play a role in regulating platelet responses is the enzyme phosphatidylinositol 3-kinase (PI3-kinase) and its lipid products PtdIns(3,4,5)P3 and PtdIns(3,4)P2. However, very little is known about exactly how PI3-kinase and its products regulate the platelet responses. Our research studies aim to gain a deeper understanding into the molecular mechanisms of PI3-kinase signal transduction in platelets, through the identification and characterization of novel platelet proteins that bind to PI3-kinase lipid products, and to define what role these proteins play in platelet PI3-kinase dependent responses.