Serum Proteome Analysis of Oral Cancer Metastasis

描述(申请人提供)：肿瘤转移是癌症患者的主要死亡原因，包括口腔鳞癌患者。然而，肿瘤转移的分子和细胞机制仍不清楚。识别与口腔鳞癌转移过程相关的分子标志物将有助于了解生物学机制，促进口腔鳞癌患者治疗干预措施的发展。这项建议旨在验证血清蛋白质组标志物可用于对非转移性口腔癌的转移进行分层的假设。有两个具体的目标来检验这一假设。目的1是利用定量蛋白质组学平台iTRAQ和二维LC-MS/MS，利用与舌鳞状细胞癌淋巴结转移相关的血清标志性蛋白。目的2是对目标1中发现的候选标记物进行AQUA验证，并建立舌鳞癌淋巴转移的多标记物预测模型。该提案的总体目标是发现血清蛋白质组标志物，然后将蛋白质组学发现转化为预测模型，可以预测原发性舌癌的淋巴转移潜力，从而改善口腔舌癌的临床治疗决策。本研究将为舌癌患者的快速筛查和淋巴结转移评估寻找血清蛋白生物标志物，提高口腔舌癌患者的临床治疗水平。

DESCRIPTION (provided by applicant): Tumor metastasis is the dominant cause of death in cancer patients, including oral squamous cell carcinoma (OSCC) patients. However, the molecular and cellular mechanisms underlying tumor metastasis are still elusive. The identification of molecular markers associated with the metastatic process would help understand biological mechanisms and facilitate the development of therapeutic interventions for OSCC patients. This proposal aims to test the hypothesis that serum proteomic markers can be used to stratify metastatic from non-metastatic oral cancers. Two specific Aims are in place to test the hypothesis. Aim 1 is to engage a quantitative proteomics platform, iTRAQ and 2-D LC-MS/MS, to harness serum signature proteins associated with lymph node metastasis of tongue SCC. Aim 2 is to conduct AQUA validation of the candidate markers discovered in Aim 1 and build multi-marker prediction models for lymph node metastasis of tongue SCC. The overarching goal of the proposal is to discover serum proteomic markers and then translate the proteomic discoveries into prediction models that can forecast lymph node metastatic potential of primary tongue cancer, and thus improve clinical treatment decisions in the management of oral tongue cancer. This proposal will discover serum protein biomarkers for fast screening and assessment of lymph node metastasis of tongue cancer and improve clinical treatment of oral tongue cancer patients.