Novel Instrumentation for Nitric Oxide Measurements in Biological Samples

描述（由申请人提供）：在该小型企业创新研究I期项目中，洛斯加托斯研究（LGR）提出开发和测试一种新型中红外激光NO分析仪，用于在宽动态范围（从皮摩尔到微摩尔）内测量细胞介质（生物样品）中的一氧化氮浓度，并具有高灵敏度（皮摩尔）。该仪器将基于LGR的专利离轴ICOS技术。在第一阶段的工作中，LGR正在与亚拉巴马亨茨维尔大学（UAH）合作开发、部署和测试这种新型便携式仪器。系统性能将首先在LGR的重量法制备的气体样品中得到证明，随后在UAH的含水生物样品中得到证明。将通过与传统化学发光分析仪（LGR）和电化学探针（UAH）直接比较来验证结果。

DESCRIPTION (provided by applicant): In this Small Business Innovative Research Phase I project, Los Gatos Research (LGR) proposes to develop and test a novel mid-infrared laser-based NO Analyzer for measurements of nitric oxide concentration in cellular media (biological samples) over a wide dynamic range (from picomole to micromole) and with a high sensitivity (picomole). The instrument will be based on LGR's patented Off-Axis ICOS technology. In this Phase I effort, LGR is collaborating with the University of Alabama Huntsville (UAH) to develop and deploy and test this novel portable instrument. System performance will be demonstrated first in gravimetrically prepared gas samples at LGR and subsequently in aqueous biological samples at UAH. The results will be validated by direct comparison with conventional chemiluminescence analyzers (at LGR) and electrochemical probes (at UAH).