Core--Gene expression profiling

广泛的基因组测序努力和微阵列技术的进步汇聚在一起，为基因表达的高通量分析创造了革命性的新机会。在该计划的背景下，基因表达分析将提供关键的信息 亨廷顿蛋白和DNA结合治疗药物改变基因的机制 表情。基因表达分析核心将通过以下方式支持该方案的个别项目 在各自的实验中跟踪数千个mRNA的同时表达 系统，从而识别特定病理或药理学的特定基因靶点 操纵。识别受特定效应影响的基因启动子将允许启动子 导致这一变化的因素和转录因子(S)有待阐明。《基因》 Expression Core将提供微阵列图谱研究的设计、执行和分析方面的专业知识，并将使用诸如Northern blotting等独立方法进行验证性分析。核心中心的首席研究员在基于微阵列的表达分析方面拥有丰富的经验，包括之前对多聚谷氨酰胺病小鼠模型的几项研究。

Extensive genome sequencing efforts and advances in microarray technology have converged to create revolutionary new opportunities for high-throughput analyses of gene expression. Within the context of the Program, gene expression analyses will provide information critical to elucidating the mechanisms by which huntingtin and DNA-binding therapeutic agents alter gene expression. The Gene Expression Profiling Core will support the Program's individual Projects by tracking the expression of thousands of mRNAs simultaneously in the respective experimental systems, thereby identifying the specific gene targets of a given pathological or pharmacologic manipulation. Identifying the gene promoters subject to a particular effect will allow the promoter elements and transcription factor(s) responsible for the change to be elucidated. The Gene Expression Core will provide expertise in the design, execution and analysis of microarray profiling studies and will carry out confirmatory analyses using independent methodologies, such as northern blotting. The Principal Investigator of the Core has extensive experience in microarray based expression analyses, including several previous studies of mouse models of polyglutamine disease.