Core--IMMUNOGENETICS OF HUMAN DIABETES

核心B设施的目标是为大规模、高分辨率遗传图谱、DNA测序和最先进的蛋白质组学提供专业知识和最先进的设施。该核心设施将为该计划中的所有项目提供人类白细胞抗原DQ和人类白细胞抗原DR基因分型，并将对除人类白细胞抗原基因以外的1型糖尿病候选基因进行高通量SNP基因分型(项目4)。提示性结果将在其他项目中输入以供确认。我们还将为GAD65单抗的鉴定(项目2)和蛋白质组学分析提供测序服务，以鉴定1型和1.5型糖尿病的抗原蛋白(项目3)。这些高通量技术将帮助计划项目调查人员确定有助于预测和治愈1型糖尿病的遗传、环境和免疫学特征。

The objective of the Core B facility is to provide expertise and a state of the art facility for large scale, high-resolution genetic mapping, DNA sequencing and state of the art proteomics. The core facility will provide HLA-DQ and HLA-DR typing for all the projects in the program and will perform high throughput SNP genotyping on type 1 diabetes candidate loci other than HLA genes (project 4). Suggestive results will be typed in other projects for confirmation. We will also provide sequencing services for the characterization of GAD65 monoclonal antibodies (project 2) and proteomics analysis for the identification of antigenic proteins in type 1 and type 1.5 diabetes (project 3). These high throughput technologies will aid the program project investigators to determine the genetic, environmental and immunological features which can help predict and cure type 1 diabetes.