COMBINED SAXS & NMR TO STUDY THE STRUCTURE & DYNAMICS OF LARGE RNA MOLECULES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 只有不到2%的人类基因组是蛋白质编码的，但超过45%的基因组转录成了RNA。越来越多的证据表明，大量未翻译的非编码(NC)RNA在生物过程中发挥的作用比人们之前认为的要广泛得多。与RNA在生物过程中所起的作用相比，我们对RNA的三维结构的了解是有限的，因为它们很难结晶，而且具有正常功能大小的RNA太大，无法用溶液核磁共振来确定结构。已知溶液X射线散射可以指示生物大分子的整体形状。最近，我们团队开发了一种组合方法，其中我们结合了内部核磁共振测量、基于同步加速器的溶液X射线散射、生化探测和计算方法，以解析溶液中大RNA(E 100 NT)的高分辨率结构。这种方法的优点在于，核磁共振和X射线散射是相辅相成的，所有的测量都可以在与生理条件有关的同一溶液条件下进行。我们的团队对ncRNAs和RNA复合体的高分辨溶液结构和动力学感兴趣。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Less than 2% of the human genome is coded for proteins, yet more than 45% of the genome is transcribed into RNA. Increasing amount of evidence indicates the vast untranslated non-coding (nc) RNAs play much wider role in biological process than previously believed. In contrast to the roles that RNA is playing in biological processes, our knowledge about the three-dimensional structures of RNA is limited because they are difficult to crystallize and RNAs with normal functional sizes are too large for structure determination using solution NMR. Solution X-ray scattering are known indicative of overall shape of bio-macromolecules. Recently, our group have developed a combined approach in which we incorporate in-house NMR measurements, synchrotron-based solution X-ray scattering, biochemical probing and computational methods to resolve high-resolution structures for large RNAs (e 100 nt) in solution. The advantage of this approach lies in the facts that NMR and X-ray scattering complement each other and all measurements can be carried out in the same solution condition which is relevant to physiologic condition. Our group is interested in the high-resolution solution structures and dynamics of ncRNAs and RNA complexes.