REACTIVE OXYGEN AND NITROGEN SPECIES IN RAW 2647 MACROPHAGE CELLS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 活性氧（ROS）是建立有效的免疫应答所必需的，但ROS也会对宿主蛋白产生有害的氧化修饰。 以前我们证明ROS诱导宿主蛋白质的氧化修饰并诱导修复过程（1）。 因此，识别蛋白质丰度的变化以及炎症（例如衰老或感染）期间的修饰，将进一步了解ROS如何促进疾病过程。 此外，表征蛋白质复合物的变化将有助于确定靶向治疗干预的重要途径。 我们的最终目标是确定细胞过程中的变化和蛋白质组的基础上炎症，确定氧化修饰蛋白质由于ROS，并确定蛋白质复合物的改变，使用高分辨率分离和高性能质谱。 这些发现将有助于确定炎症性疾病和相关病理的根本原因，最终目标是开发治疗干预（例如药理学治疗）以遏制疾病进程。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reactive oxygen species (ROS) are required for mounting an effective immune response, yet ROS also produce detrimental oxidative modifications to host proteins. Previously we demonstrated that ROS induce oxidative modifications to host proteins and induce repair processes (1). Thus, the identification of changes in protein abundance as well as modifications during inflammation (e.g. aging or infection), will further our understanding of how ROS contribute to disease processes. In addition, characterization of changes in protein complexes will aid in the identification of vital pathways for targeted therapeutic intervention. Our ultimate goals are to identify changes in cellular process and the proteome based upon inflammation, determine oxidative modifications to proteins due to ROS, and determine alterations in protein complexes using high resolution separations and high performance mass spectrometry. These findings will be instrumental in defining the underlying cause of inflammatory disease and associated pathologies with the end goal of development of therapeutic interventions (e.g. pharmacological therapeutics) to curb disease processes.