INTERACTION OF HUMAN PROFILIN W/PHOSPHATYDILINOSITOL IN GUVS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们将研究BODIPY TMR标记的PIP 2在含有不同比例PIP 2（1%-16%）的POPC制成的巨单层囊泡（GUV）中的动力学，使用扫描FCS和数量和亮度分析。我们还将通过测量LAURDAN的GP来研究PIP 2的横向组织，所述LAURDAN的GP在GUV中在存在和不存在各种PIP 2浓度的人profilin的情况下被2光子激发。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We will investigate the dynamics of BODIPY TMR labelled PIP2 in Giant Unilamellar vesicles (GUV) made of POPC with various proportion of PIP2 (1%-16%) using scanning FCS and Number and Brightness analysis. We will also study the lateral organisation of the PIP2 by measuring the GP of LAURDAN following 2 photon excitation in the GUV in presence and absence of Human profilin for various PIP2 concentrations.