MURINE PELVIC ANATOMY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 可见小鼠与呼吸机共享的数据 我们对泌尿生殖系统疾病感兴趣，并已开始对我们的病理标本进行MR显微镜检查。 我们发现对正常解剖结构的详细研究很少，我们认为在这种情况下使用通用数据集是有意义的。 杜克"可见老鼠"的提法对我们很有吸引力。 因此，我们求助于杜克大学的CIVM，要求获得雄性和雌性的数据集，因为已发表的C57 B16小鼠的图像显然是精致的。 C57B16/J、FVB、CRL-CD 1是常用的，我们将感兴趣。 我们的重点是在病理学研究之前对组织样本进行详细的高分辨率MR显微镜检查。我们还没有做过整个动物甚至整个骨盆的正常动物。我们在一个蜂群中发现了显性突变导致了盆腔器官脱垂。我们正试图确定这一偶然发现是否值得进一步研究，我们需要正常小鼠解剖学的例子进行比较。 我们将尝试生成相关盆腔解剖结构的标记图像。 脱垂模型是由Aimee约翰逊（Aimee Johnson）在一篇论文中发现的，她是Jay里德博士在病理学方面的博士生;他们两人都是我在多个项目中的合作者。 我在泌尿妇科专科的同事听说了这个模型，他们变得非常感兴趣，因为脱垂是一个重要的临床问题（Gunhilde Buchsbaum，Michael Flynn，安娜·麦克南利）。 我们一直与Scott Kennedy在生物化学和生物物理学方面合作，他是我们真正的MR显微镜人才。 我们是经验丰富的ImageJ和Amira用户。Amira是我们的首选工具，一旦数据集进行初始操作。 我们已经发表了一些微CT工作的下尿路与R。宁 对照数据集显然比推动这一初步调查的因素具有更广泛的意义，并且将引起更广泛受众的兴趣。 我们与两位兽医病理学家Raymond巴格斯博士和Cynthia Besch-Williford博士（分别来自俄勒冈州和密苏里州）进行了合作，我们经常向他们寻求特殊的治疗方法。 专业知识 在磁共振显微镜的兴趣正在增长，在我们的机构，资源是有限的。如果需求增加或无法解决具体的技术问题，我们可能需要求助于CIVM进行成像 问题研究

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DATA OF VISIBLE MOUSE SHARED WITH INVESTIGATOR We are interested in disorders of the genitourinary system, and have commenced doing MR microscopy of our pathology specimens. We are finding very little detailed study of normal anatomy, and we think it makes sense to use a common dataset in this context. The Duke "visible mouse" reference is very appealing to us. Hence we are turning to the Duke CIVM to request access to male and female datasets, as the published images of C57Bl6 mice are clearly exquisite. C57Bl6/J, FVB, CRL-CD1 strains are commonly used and would be of interest to us. Our focus has been detailed high-resolution MRmicroscopy of tissue samples prior to pathology studies. We have not done whole animal or even whole pelvis normal animals at this point. We have encountered what appears to be a dominant mutation in one of our colonies resulting in pelvic organ prolapse. We are attempting to determine if this serendiptous finding warrants further study, and we need examples of normal murine anatomy for comparison purposes. We would attempt to generate labelled images of the pertinent pelvic anatomy. The prolapse model was detected in a thesis by Aimee Johnson, a doctoral student of Dr. Jay Reeder in Pathology; both are my collaborators on multiple projects. My colleagues in the division of urogynecologic specialties heard of this model, they became very interested as prolapse is a significant clinical problem (Gunhilde Buchsbaum, Michael Flynn, Anna McNanly). We have been collaborating with Scott Kennedy in Biochemistry and Biophysics, our true MR microscopy talent. We are experienced ImageJ and Amira users.. Amira being our preferred tool once the data set undergoes initial manipulation. We have published some microCT work of the lower urinary tract with R. Ning. The control dataset is clearly of broader significance than what is driving this initial investigation, and will be of interest to a wider audience. We have collaborations in place with two veterinary pathologists, Dr. Raymond Baggs and Dr. Cynthia Besch-Williford (Oregon and Missouri, respectively) to whom we routinely turn for their special expertise. Interest in MR microscopy is growing at our institution, and the resource is currently limited. If demand increases or specific technical problems cannot be addressed, we may need to turn to CIVM for the conduct of imaging studies.