Shared Resources-Microarray

微阵列共享资源（MSR）于1999年启动，旨在快速、经济高效地提供 微阵列技术对癌症研究的影响。MSR包含一个功能齐全的斑点微阵列 该设备具有3，548 ff空间，并打印cDNA，寡核苷酸和蛋白质载玻片微阵列。 100级洁净室中最高的质量。点状阵列服务包括： i）全基因组和靶向人和小鼠斑点DNA微阵列和方案的可用性， ii）完整的项目服务，包括标记、杂交、扫描和原始数据分析， iii）为非标准应用程序专门化、研究者定义的打印项目提供定制阵列服务， iv）为商业上可获得的蛋白质微阵列（例如Clontech Ab 500）提供完整的项目服务，以及 定制打印蛋白质。调查人员能够使用任何或所有提供的工具和服务， 需要.自成立以来，已有68名YCC成员使用了MSR点样微阵列服务。这 占所有癌症中心成员的22%。在2005年，19名YCC调查员占18% 的3,550个斑点载玻片微阵列提供服务。 MSR中包含的Affychip基因芯片部分成立于2001年， 利用基于AffyIdea寡核苷酸的微阵列进行大规模基因表达研究。统称 基因芯片和斑点载玻片微阵列技术提供了全面的微阵列支持， 癌症研究有可能帮助确定疾病的生化原因， 更好的诊断方法，并改善治疗结果和患者护理。基因芯片部分 MSR占地1,753平方英尺，是新建造的，定制设计的实验室空间，设备齐全 使用基因芯片仪器系统和ABI序列检测系统。提供Affyoung服务 包括i）表达分析，其包括RNA/mRNA提取、适合于 阵列杂交和数据分析，ii）使用Affyssin（10、100和500 K测定）进行SNP分析 和ABI平台，和iii）使用ABI 7900序列的微阵列结果的定量PCR验证 检测系统。MSR的载玻片微阵列和Affyssin基因芯片部分都将参考 根据需要进行实验设计、数据解释、手稿和资助申请准备，并将 提供标记、杂交和原始数据分析的培训。在2005年，25名YCC调查员 在2，334项提供的服务中，使用Affyestry基因芯片服务的占22%。自成立以来， Affyoung部门为93名耶鲁大学研究人员和30名来自其他机构的研究人员提供了6,000多项服务。

The Microarray Shared Resource (MSR) was initiated in 1999 to rapidly and cost-effectively bring the power of microarray technology to bear on cancer research. The MSR contains a fully-functional spotted microarray facility that has 3,548 ff space and prints cDNA, oligonucleotide, and protein glass slide microarrays of the highest possible quality in class 100 cleanrooms. Spotted array services include: i) availability of whole genome and targeted human and mouse spotted DNA microarrays and protocols, ii) complete project services including labeling, hybridization, scanning and primary data analysis, iii) custom array services for specialized, investigator defined, printing projects for non-standard applications, iv) complete project services for commercially available protein microarrays (e.g. Clontech Ab500) and custom printing of proteins. Investigators are able to use any or all of the tools and services offered as they require. 68 YCC members have utilized MSR spotted microarray services since its inception. This represents 22% of all Cancer Center members. During CY 2005, 19 YCC investigators accounted for 18% of the 3,550 spotted glass slide microarray provided services. The Affymetrix GeneChip section, which is included in the MSR, was established in 2001 to support large-scale gene expression studies utilizing Affymetrix oligonucleotide based microarrays. Together, the GeneChip and spotted glass slide microarray technologies offer comprehensive microarray support for cancer research which has the potential to aid in identification of biochemical causes of the disease, develop better diagnostic methods, and to improve therapeutic outcomes and patient care. The GeneChip section of the MSR occupies 1,753 ft2 of newly constructed, custom-designed laboratory space and is well equipped with GeneChip instrument systems and an ABI Sequence Detection system. Available Affymetrix services include i) expression analysis that includes RNA/mRNA extraction, preparation of target samples suitable for array hybridization, and data analysis, ii) SNP analysis using both Affymetrix (10, 100, and 500 K assays) and ABI platforms, and iii) Quantitative PCR validation of microarray results using the ABI 7900 Sequence Detection system. Both the glass slide microarray and Affymetrix GeneChip sections of the MSR will consult as needed on experimental design, data interpretation, manuscript and grant application preparation, and will provide training for labeling, hybridization and primary data analysis. During CY 2005, 25 YCC investigators using Affymetrix GeneChip services accounted for 22% of the 2,334 provided services. Since inception, the Affymetrix section has provided >6,000 services to >93 Yale investigators and 30 from other institutions.