Experimental Component

该提案的总体主题是“肿瘤异质性对癌症进展的影响”。因此，实验组件适合于量化癌细胞在癌症进展的标志性特征方面的异质性，例如增殖、代谢和运动性。我们将量化模型培养细胞系中的这种性状异质性，在几个mE扰动下，通过高含量自动显微镜和图像处理在单细胞水平上进行平均和分布测量。 性状分布也将作为统计亚群进行解析，以揭示共同趋势 在细胞系之间。几种体内肿瘤实验系统将补充体外试验，用于理论模型验证。我们已经组建了一个由才华横溢的实验人员组成的团队，他们涵盖了该组件所需的所有特定技能，正如他们杰出的出版记录所示。科学家将确保组件技术的连续性。

The overarching theme of this proposal is "Impact of Tumor Heterogeneity on Cancer Progression". The Experimental Component is therefore geared to quantifying cancer cell heterogeneity with respect to hallmark traits of cancer progression, such as proliferation, metabolism and motility. We will quantify this trait heterogeneity in model cultured cell lines, under several mE perturbation, as average and distribution measurements performed at the single-cell level by high-content automated microscopy and image processing. Trait distributions will also be resolved as statistical subpopulations, in order to uncover common trends between cell lines. Several In vivo tumor experimental systems will complement in vitro assays for the purpose of theoretical model validation. We have assembled a team of talented experimentalists that cover all specific skills needed for this Component, as shown by their distinguished publication records. Staff scientists will assure continuity of Component technology.