REDOX REGULATION OF HYPOXIA INDUCIBLE FACTOR (HIF)-1 IN CEREBRAL ISCHEMIA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 项目1：PI  石红莲 描述 氧和葡萄糖供应的减少涉及许多病理条件，如中风，并有助于缺血性损伤。不平衡的葡萄糖和氧气供应和代谢诱导氧化应激并改变细胞氧化还原（还原/氧化）环境。 细胞氧化还原环境对于细胞信号传导和细胞存活的调节至关重要。 氧化还原调节的分子靶点之一伊势缺氧诱导因子1，已发现其在脑缺血的细胞死亡和存活中起关键作用。 我们正在进行的项目将利用UNM BRaIN中心现有的最先进技术，通过体外和体内模型研究缺血性卒中的氧化还原变化。 我们的方法包括MRI，电子顺磁共振（EPR）光谱和成像，双光子显微镜，荧光显微镜，细胞和分子技术。 我们的目标是发现潜在的有效和安全的中风治疗方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Project 1: PI  Honglian Shi Description Reduced supply of oxygen and glucose involves in many pathological conditions such as stroke and contributes to ischemic injuries. Imbalanced glucose and oxygen supplies and metabolism induces oxidative stress and alters cellular redox (reduction/oxidation) environment. Cellular redox environment is critical for the regulation of cellular signaling and cell survival. One of the molecular targets of redox regulation ise hypoxia inducible factor 1, which has been found to play a critical role in cell death and survival in cerebral ischemia. Our ongoing project will utilize state of the art technology available in UNM BRaIN center to study redox changes in ischemic stroke with in vitro and in vivo models. Our approaches include MRI, electron paramagnetic resonance (EPR) spectroscopy and imaging, two-photon microscopy, fluorescence microscopy, and cellular and molecular techniques. Our goal is to discover potential effective and safe approaches for stroke treatment.