Multicolor Fluorescent Analysis in Zebrafish

描述（由申请人提供）：斑马鱼胚胎和幼虫的半透明使它们非常适合用于发育和生理过程的体内分析。本研究旨在开发用于斑马鱼细胞多色荧光标记的工具。先前的研究表明，随机DNA重组可导致不同细胞中荧光蛋白的不同组合表达。荧光蛋白的组合表达赋予细胞许多颜色，从而提供了一种区分相邻细胞和可视化细胞相互作用的方法。我们将开发工具，使该技术广泛而容易地应用于斑马鱼发育和解剖研究（目标1），并生成下一代试剂和方案，使该技术能够在斑马鱼生物学的各个方面得到复杂的应用（目标2）。

DESCRIPTION (provided by applicant): The translucency of zebrafish embryos and larvae makes them ideally suited for the in vivo analysis of developmental and physiological processes. This study aims to develop tools for the multicolor fluorescent labeling of zebrafish cells. Previous studies have demonstrated that stochastic DNA recombination can lead to the expression of different combinations of fluorescent proteins in different cells. The combinatorial expression of fluorescent proteins endows cells with many colors, thereby providing a way to distinguish adjacent cells and visualize cellular interactions. We will develop tools that make this technology widely and easily applicable to the study of zebrafish development and anatomy (Aim 1) and generate next-generation reagents and protocols that allow the sophisticated application of this technology to various aspects of zebrafish biology (Aim 2). PUBLIC HEALTH RELEVANCE: Zebrafish is a vertebrate model organism that shares many biological processes with humans. Research in zebrafish has provided important insights into the causes of birth defects and disease. The proposed work is to enhance the utility of this model system by developing tools to label cells in multiple different colors and follow their development.