HORMONE ASSAY CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 荷尔蒙分析核心实验室是在获得资金后开发的儿童权利公约的第一个组成部分。荷尔蒙分析核心实验室的目标是通过开发和提供研究人员目前无法获得的荷尔蒙测量方法，来扩大查尔斯·德鲁大学的荷尔蒙测试能力。 荷尔蒙分析核心实验室在改进现有分析以满足研究人员的特定需求以及开发新的分析方面取得了相当大的成功。以下是目前可用的激素检测方法列表： 1.血清黄体生成素：双位点荧光免疫测定法。 2.血清FSH：双位点荧光免疫分析(FIA)。 3.睾酮：睾酮的测定使用碘化示踪剂，并在进行免疫分析之前，使用防己烷：乙酸乙酯(2：3)混合溶剂提取血清样本。该实验室已经发布了一种高度敏感的睾酮测试的开发，该测试已经为测量女性的低睾酮水平进行了优化。 4.游离睾酮：采用直接平衡透析法测定游离睾酮水平。该实验室已经发布了用于测量女性低游离睾酮水平的灵敏分析的开发和验证，以及月经女性标准数据的生成。 5.二氢睾酮：血清样品通过Celite色谱柱提取和层析。用放射免疫分析法测定Cellite组分的洗脱液中的DHT浓度。 6.性激素生成球蛋白(SHBG)：采用示踪量[~3H-DHT]和DHT标准系列稀释法测定血清SHBG结合量。 7.胰岛素：胰岛素测定使用Wellcome的标准相当于第一个1RP GG/304，ICN的抗猪抗体和礼来公司的猪胰岛素用于碘标记。 8.C-肽：C-肽分析使用由礼来公司提供的抗血清、标准品和碘化材料以及乙醇分离技术。雄烯二酮、脱氢表雄酮、雌酮、雌二醇、肌肉生长抑素、炎症标记物、C反应蛋白和IL-6。 9.自最初的资助以来，HAC实验室提供了最先进的服务，并在开发新的分析方法方面具有很高的创新性。HAC实验室鼓励分子生物学家和临床研究人员之间的合作。HAC实验室取得了卓越的成就；它支持了大量影响大、经过同行评审的出版物和新的RO1赠款申请。 注：CORE不进行任何S自己的研究。它为获得CRC和IRB批准的研究提供激素测量。因此，它使用与CRC总括拨款相同的IRB批准。CORE没有患者登记。它纯粹是一个激素测量实验室。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Hormone Assay Core Lab was the first component of the CRC to be developed following funding. The objective of the Hormone Assay Core Lab is to expand the hormone assay capabilities at Charles Drew University by developing and making available hormone measurement methods not currently available to the investigators. The Hormone Assay Core Laboratory has had considerable success in refining existing assays to meet specific needs of investigators, and in developing new assays. Below is a list of hormone assays that are currently available: 1. Serum LH: A 2-site directed fluoroimmunometric assay (FIA). 2. Serum FSH: A 2-site directed fluoroimmunometric assay (FIA). 3. Testosterone: The assay for testosterone uses an iodinated tracer and extraction of serum samples using anexane: ethyl acetate (2:3) mixture, prior to immunoassay. The laboratory has published the development of a highly sensitive testosterone assay that has been optimized for the measurement of low testosterone levels in women. 4. Free Testosterone: Free testosterone levels are measured by a direct equilibrium dialysis procedure. The laboratory has published the development and validation of the sensitive assay for the measurement of low free testosterone levels in women, and the generation of normative data in menstruating women. 5. Dihydrotestosterone: Serum samples are extracted and chromatographed through a Celite column. DHT concentrations are measured in the eluate from the Celite fractions by RIA. 6. Sex-Hormone Building Globulin (SHBG): Serum SHBG binding capacity is measured by using tracer amounts of [3H-DHT] and serial dilutions of DHT standards. 7. Insulin: Insulin assay uses a standard from Wellcome equated to 1st 1RP GG/304, antiporcine antibody from ICN and porcine insulin from Lilly for iodination. 8. C-Peptide: C-Peptide assay uses antisera, standards and iodination material supplied through the Eli Lilly Company and an ethanol separation technique. Androstenedione, DHEA, Estrone, estradiol, myostatin, inflammatory markers, C-reactive protein and IL-6. 9. Since its initial funding, the HAC Lab has provided state-of-the-art service, and has been highly innovative in developing new assays. The HAC Lab has encouraged collaborations between molecular biologists and clinical investigators. The HAC Lab has had an outstanding record of accomplishments; it has supported a large number of high-impact, peer-reviewed publications, and new RO1 grant applications. Note: The Core does not conduct any studies of it''s own. It provides hormone measurements to studies that are CRC and IRB approved. Therefore, it uses the same IRB approval as the umbrella CRC grant does. There is no patient enrollment by the Core. It is purely a hormone measurement laboratory.