Flow Cytometry & Cell Sorting

霍林斯癌症中心(HCC)流式细胞仪和细胞分选(FCCS)共享资源为肝癌提供 成员全面的分析流式细胞仪和最先进的高速细胞分类服务 经济实惠的方式。在医学博士周道红的领导下，FCC配备了一台Becton- Dickinson FACSCalibur分析流式细胞仪、Becton-Dickinson FACSAria细胞分选仪和DAKO细胞分选仪 VIoFlo高速细胞分选器。该资源的工作人员在高速分拣方面拥有相当多的专业知识 包括干细胞/祖细胞和表位特异性T细胞在内的稀有细胞群体，并不断 扩大现有技术库，以满足创新研究基地的需要 肝细胞癌。 自2003年以来，肝细胞癌FCC共享资源已经支持了123篇出版物的研究 由33名代表所有肝细胞癌项目和华盛顿医科大学八个部门的调查人员 南卡罗来纳州。肝细胞癌FCC共享资源在几个开创性的 在这段时间里，肝细胞癌成员所做的发现。控制和控制中心的具体目标 共享资源将用于： ？提供多参数和多激光测量，包括：细胞凋亡、细胞 增殖，细胞周期分布，免疫表型以及细胞内的检测 蛋白质、膜扰动和生物活性分子 提供基于细胞表面标记免疫染色和/或侧群的高速细胞分选 绿色荧光蛋白的染色和表达 ？提供有关实验设计、样品制备、数据的咨询和帮助 分析和编制与出版物和赠款申请有关的高质量数字 流式细胞术 ？提供有关使用常规流式细胞仪技术的培训，并提供更高级的培训 肝细胞癌委员对新开发的流式细胞仪检测的关注 ？发现和开发新的流式细胞仪技术，以协助肝癌的前沿研究

The Hollings Cancer Center (HCC) Flow Cytometry & Cell Sorting (FCCS) Shared Resource provides HCC members comprehensive analytic flow cytometry and state-of-the-art high speed cell sorting services in a cost effective manner. Under the directorship of Daohong Zhou, MD, the FCCS is equipped with a Becton- Dickinson FACSCalibur Analytical Flow Cytometer, a Becton-Dickinson FACSAria Cell Sorter and a Dako VIoFlo High-Speed Cell Sorter. The staff of the resource have considerable expertise in high-speed sorting of rare populations of cells including stem/progenitor cells and epitope-specific T cells and are continually expanding the repertoire of available techniques to meet the needs of the innovative research base within the HCC. Since 2003, the HCC FCCS Shared Resource has supported research that has resulted in 123 publications by 33 investigators representing all of the HCC programs and eight Departments at the Medical University of South Carolina. The HCC FCCS Shared Resource has played an essential role in several of the seminal discoveries made by members of the HCC during this time. The specific objectives of the HCC FCCS Shared Resource are to: ¿ Provide multi-parametric and multi-laser measurements including measurements of: apoptosis, cell proliferation, cell cycle distribution, and immunophenotyping, as well as detection of intracellular proteins, membrane perturbations, and bioactive molecules ¿ Provide high-speed cell sorting based on cell surface marker immunostaining and/or side-population staining, and expression of green fluorescent protein ¿ Provide consultation and assistance concerning experimental design, sample preparation, data analysis, and preparation of high quality figures for publications and grant applications as they relate to flow cytometry ¿ Provide training in the use of routine flow cytometric technology and offer more advanced training for HCC members concerning the newly developed flow cytometric assays ¿ Identify and develop new flow cytometric technologies to assist cutting-edge research in the HCC