IMAGE CORRELATION SPECTROSCOPY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我们希望使用图像相关光谱的新方法来确定封面幻灯片上每平方微米的荧光团的数量密度。我们的实验包括将DNA分子嫁接到玻璃盖片上。然后，含有合适分子的互补DNA链与玻璃上的DNA分子杂交。我们推测，嫁接的DNA分子的密度约为每平方米100微米。用羧基荧光素标记DNA作为荧光标记物。我们想学习图像相关光谱技术，这将需要一个低背景的荧光设置资源与其倒置显微镜。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We would like to determine the number density of fluorophores per square micrometer on a cover slide using the novel approach of image correlation spectroscopy. Our experiments involve grafting DNA molecules on glass coverslips. Complementary DNA strands containing a fitting molecule are then hybridized to the DNA molecules on the glass. We guess that the density of grafted DNA molecules is about 100 per square micrometer. The DNA is labeled with carboxy-fluorescein as fluorescent marker. We would like to learn the technique of image correlation spectroscopy which would require a low-background fluorescence set up the Resource has with its inverted microscope.