INVESTIGATING THE REACTIVITY OF PLANT CELL WALL COMPONENTS

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 2D NMR实验，使用梯度-HSQC 1-键13 C-1H相关性，对来自裸子植物和被子植物的非衍生化植物细胞壁材料进行研究，揭示了主要的细胞壁组分，从而可以通过高分辨率溶液状态NMR分析整个细胞壁。我们目前正在研究各种细胞壁成分的反应性，利用几种衍生化方法，了解植物细胞壁内的缩聚反应机制。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. 2D NMR experiments, using gradient-HSQC 1-bond 13C-1H correlation, on non-derivatized plant cell wall material from gymnosperms and angiosperms reveal the major cell wall components, allowing the whole cell wall to be analyzed via high-resolution solution-state NMR. We are currently investigating the reactivity of various cell wall components utilizing several derivatization methods towards understanding polycondensation reaction mechanisms within the plant cell wall.