BINDING PARTNERS FOR BTB-DOMAIN-CONTAINING PROTEIN YIL001W

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 BTB结构域是存在于所有真核生物中的蛋白质-蛋白质相互作用结构域。 人类细胞中许多含有BTB结构域的蛋白质是泛素连接酶的衔接子。作为泛素连接酶接头蛋白，BTB蛋白将催化RING蛋白桥接至靶向泛素化的蛋白。 在酵母中，Yil 001 w符合BTB泛素连接酶衔接子的描述。 我们发现Yil 001 W在一个小的Y2 H筛选中与一个剔除蛋白Cul 3相互作用。 我们有兴趣为Yil 001 w寻找更多的结合伴侣，以尝试将推定的泛素连接酶融入细胞途径。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The BTB domain is a protein-protein interaction domain that is present in all eukaryotes. Many BTB-domain-containing proteins in human cells are adaptors for ubiquitin ligases. As a ubiquitin ligase adaptor protein, the BTB protein bridges the catalytic RING protein to the protein that is targeted for ubiquitination. In yeast Yil001w fits the description of a BTB ubiquitin ligase adaptor. We found that Yil001W interacts with a cullin, Cul3, in a small Y2H screen. We are interested in finding more binding partners for Yil001w to try to fit the putative ubiquitin ligase into a cellular pathway.