PROTEIN/SIRNA COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 RNA沉默是一种古老的以RNA为基础的免疫反应，从分裂酵母到人类都是保守的。为了抗病毒感染，宿主利用结构独特的小RNA与感染病毒的mRNAs配对进行降解。然而，病毒本身在数百万年的进化过程中产生了各种各样的RNA沉默抑制子来对抗抗病毒反应。这一建议的主要目标是在分子水平上研究通过自然选择进化出来的宿主和病毒之间的这种反相互作用。 项目1 人甲型流感病毒NS1蛋白识别dsRNA的结构基础 甲型流感病毒是导致周期性大流行威胁的重要人类病原体，而甲型流感病毒(NS1A)的NS1蛋白保护病毒免受宿主的防御。我们计划解决Ns1A/siRNA双链的复杂结构。 项目2 番茄无精症病毒蛋白2b抑制RNA沉默的结构基础 黄瓜病毒编码的2B蛋白在感染过程中作为转录后基因沉默(PTGS)抑制子来对抗寄主防御。我们计划解决Tav2b/siRNA双链的复杂结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. RNA silencing is an ancient RNA-based immune response, which is conserved from fission yeast to human beings. In order to anti-virus infection, host uses structural unique small RNAs to pair with the infected virus mRNAs for degradation. However, virus itself has produced diversified RNA silencing suppressors to counter anti-virus response during millions years evolution. The main goal of this proposal is to study this counter-interaction between the host and virus evolved by nature selection at the molecular level. Project 1 Structural Basis for dsRNA Recognition by NS1 protein of Human Influenza Virus A Influenza A viruses are important human pathogens resulting in periodic pandemic threaten, while NS1 protein of influenza A virus (NS1A) shields the virus against host defense. We plan to solve the complex strcuture of Ns1A/siRNA duplex. Project 2 Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b 2b protein encoded by cucumovirus functions as post-transcriptional gene silencing (PTGS) suppressor to counter host defense during infection. We plan to solve the complex structure of Tav2b/siRNA duplex.