INVESTIGATION OF THE [4FE-4S] CLUSTER IN APS REDUCTASE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 最近的结晶学表征表明，在硫酸盐还原酶APS还原酶的活性位置上有独特的[4Fe-4S]簇配位。然而，结构的适度分辨率(2.7°)不允许详细分析簇的几何结构，这包括两个不变的相邻半胱氨酸残基的配位。XAS将用于提供APS还原酶中唯一的[4Fe-4S]簇的详细局部结构信息。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent crystallographic characterization suggests unique [4Fe-4S] cluster coordination in the active site of the sulfatereducing enzyme, APS reductase. However, the modest resolution of the structure (2.7 ¿) does not permit detailed analysis of cluster geometry, which includes coordination by two invariant adjacent cysteine residues. XAS will be used to provide detailed local structure information for the unique [4Fe-4S] cluster in APS reductase.