ASSEMBLY OF THE 30S RIBOSOMAL SUBUNIT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 A)细菌30S核糖体由1542个核苷酸、16S核糖体RNA和20个小核糖体蛋白组成。在蛋白质合成过程中，它负责与50S亚基相关的mRNA解码。大肠杆菌30S亚基可在体外从纯化的组分中重组。已开发出包含平行蛋白质结合和顺序蛋白质结合作为机制特征的组装图。 30s组装的新兴图景是，它是组装路径的统计分布，但比起组装版图，有更受欢迎的路线。与蛋白质结合的亚组的中间体在组装过程中积累，但这些中间体最终会聚在最终完成的亚基上。我们感兴趣的是通过在体外重建过程中可视化大量的核糖体颗粒来测量中间体种群的时间依赖性。 B)30S亚基是自发形成分子组装的范例。无论是从结构上还是从组装的角度来看，它都是最具特点的大型系统之一。了解30S组装的详细机制将为许多类别的分子机器的组装提供洞察力，涉及大量组件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A) The bacterial 30S ribosome is composed of a 1542 nucleotide 16S ribosomal RNA and 20 small ribosomal proteins. It is responsible for mRNA decoding in association with the 50S subunit during protein synthesis. The 30S subunit from E. coli can be reconsituted in vitro from purified components. An assembly map has been developed that contains both parallel protein binding and sequential protein binding as mechanistic features. The emerging picture of 30S assembly is that it is a statistical distribution of assembly pathways, but that there are favored routes over the assembly landscape. Intermediates with subsets of proteins bound accumulate during the assembly, but these intermediates eventually converge on the final completed subunit. We are interested in measuring the time dependence of the populations of intermediates by visualizing large number of ribosomal particles during in vitro reconstitution. B) The 30S subunit is a paradigm for spontaneous formation of a molecular assembly. It is one of the best characterized large systems both structurally and from the standpoint of assembly. Understanding the detailed mechanism of 30S assembly will provide insights into the assembly of many classes of molecular machines involving large numbers of components.