NOVEL 2D,3D MORPHOMETRIC INDICATORS IN DYSPLASIA IN BARRETT'S ESOPHAGUS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 萨博博士在2005年12月获得了基于竞争性RFA的Cobre基金。这笔资金于2006年2月启动。 背景：Barrett‘s食道上皮异型增生分级为治疗决策提供依据。评分存在相当大的观察者间变异性。我们开发了一个客观的形态计量学模型来区分不同级别的异型增生(结果在USCAP 2006会议上公布)。 新的目标：1.将形态计量学结果与Barrett‘s食道患者从不典型增生进展到浸润性癌的时间相关联。我们的初步研究表明，质地最能区分不典型增生的级别。由于质地反映染色质的分布，我们假设质地的量化可能有助于预测从不典型增生到浸润性癌的进展。2.利用激光捕获显微切割和基因芯片技术，将形态计量学模型与这些细胞的遗传图谱进行关联。当基因签名将与形态计量学相关时，具有预测性和或 治疗潜力可能会被开发出来。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dr. Sabo was awarded COBRE funding based on a competitive RFA in Dec. 2005. The funding was activated in Feb. 2006. Background: Grading of epithelial dysplasia in Barrett's esophagus provides the basis for treatment decisions. Grading suffers from considerable interobserver variability. We developed an objective morphometric model to differentiate between grades of dysplasia (results presented at USCAP 2006 conference). New goals: 1.To correlate the morphometrical results with time of progression from dysplasia to invasive carcinoma in Barrett's esophagus patients. Our preliminary study showed texture to best differentiate between dysplasia grades. Since texture reflects chromatin distribution, we hypothesized that quantifying texture may help to predict progression from dysplasia to invasive carcinoma. 2.To correlate between the morphometrical model, and the genetic profile of these cells, using laser capture microdissection and gene microarray technology. When gene signatures will be correlated with morphometry, novel molecular markers with predictive and or therapeutic potentials may be developed.