Flow

梅奥临床肿瘤中心(MCCC)的流式细胞术/光学形态学共享资源 提供与这两个领域相关的工具、专业知识和培训。流式细胞术用于高速 液体悬浮液中细胞的分析和分选。光学形态是指相关的技术 用光学显微镜和图像分析。流式细胞仪区域有各种不同的仪器 从单激光台式分析系统到五激光四路细胞分选机。调查人员， 学生和技术人员可以选择将准备好的样品送到资源人员手中 或接受过操作五种分析流式细胞仪中的任何一种的培训。资源人员执行单元格 分类。光学形态区域容纳了各种基于光学显微镜的仪器，包括激光 扫描共焦显微镜和其他配备专门的直立和倒置光学显微镜 用于光学切片、微注射、比率成像和全内反射的部件 荧光法(TIRF)。所有这些显微技术都提供了培训。图像分析软件 此外，还提供定制的宏编程以及适当的培训。资源 人员可协助培训、故障排除、维护、数据解释和 实验设计。

The Flow Cytometry/Optical Morphology Shared Resource of the Mayo Clinic Cancer Center (MCCC) provides instrumentation, expertise, and training related to these two areas. Flow cytometry is used for highspeed analysis and sorting of cells in liquid suspension. Optical morphology refers to techniques associated with light microscopy and image analysis. The flow cytometry area houses instruments of varying capabilities from a single laser bench-top analytical system to a five-laser, four-way cell sorter. Investigators, students, and technicians have the option of dropping off prepared samples to be run by resource personnel or to be trained to operate any of the five analytical flow cytometers. Resource personnel perform cell sorting. The optical morphology area houses a variety of light microscopy based instruments including laser scanning confocal microscopes and other upright and inverted light microscopes equipped with specialized components for optical sectioning, microinjection, ratiometric imaging, and total internal reflection fluorescence (TIRF). Training is provided for all of these microscopy techniques. Image analysis software including customized macro programming along with appropriate training is also available. Resource personnel are available to assist with training, troubleshooting, maintenance, data interpretation, and experimental design.