MoFlo XDP High Speed Cell Sorter

描述（由申请人提供）：免疫系统的细胞是保护我们免受病原体入侵和提供长期免疫再感染不可或缺的。基本上，特鲁多研究所的所有15名主要研究人员都得到资助，致力于研究控制急性和长期免疫细胞对感染反应的基本机制，并探索其开发疫苗的潜力。为了研究这些细胞的特性和功能，需要根据它们独特的表型分离出不同的种群。然而，许多这些免疫细胞，如记忆T细胞亚群、树突状细胞亚群或嗜碱性细胞，是非常罕见的，只能通过许多表型标记的组合来识别。此外，越来越多地使用携带荧光标记蛋白的转基因动物排除了使用非荧光分离方法，如磁珠。因此，基于荧光参数组合阵列的高速多参数荧光激活细胞分选仪是对罕见免疫细胞进行有效分选的必要条件。虽然特鲁多研究所的所有研究人员多年来一直在使用细胞分选器，但现有仪器已有近十年的历史，并且在速度、可用荧光色的数量以及制造商的服务和部件支持方面存在严重限制，这些限制将于2010年底到期。所要求的MoFlo XDP高速细胞分选器将克服所有这些限制，并允许我们a)以显着更高的速度进行分选，这对于稀有种群的分选至关重要，并且由于缩短了分选时间而产生更多的用户时间，b)基于更独立的荧光参数进行分选，这对于识别特定的免疫细胞亚群至关重要，c)由于显着缩短了设置周期，增加了所有研究人员可用的有效分选。d)确保制造商贝克曼库尔特的持续服务和零件支持。尽管研究人员使用的个别实验和程序可能会随着时间的推移而改变，但所要求的分选机的开放式架构是由制造商设计的，以便于将来根据需要进行修改。该仪器将由流式细胞术中心的专职人员专门操作和维护。由于基本上所有研究人员的研究工作都严重依赖于高速细胞分选机，特鲁多研究所完全致力于提供所有必要的支持，以便在未来容纳和维护该仪器。

DESCRIPTION (provided by applicant): The cells of the immune system are indispensable to protect us from invading pathogens and provide long-lasting immunity to re-infection. Essentially all of the 15 principal investigators at Trudeau Institute are funded and dedicated to study the fundamental mechanisms governing acute and long-lasting immune cell responses to infection and explore their potential for vaccine development. In order to study the properties and functions of these cells it is required to isolate distinct populations based on their unique phenotype. However, many of these immune cells, such as memory T cell subsets, dendritic cell subsets or basophils, are very rare and can only be identified by a combination of numerous phenotypic markers. Moreover, the increasing use of transgenic animals carrying fluorescent marker proteins excludes the use of non-fluorescent isolation methods such as magnetic beads. For these reasons a high speed multi-parameter fluorescence activated cell sorter is indispensable to effectively sort infrequent immune cells based on a combinatorial array of fluorescence parameters. While all investigators at Trudeau Institute have been using a cell sorter for many years the existing instrument is almost a decade old and poses critical limitations with respect to speed, the number of usable fluorochromes as well as the service and parts support by the manufacturer which will expire by the end of 2010. The requested MoFlo XDP High Speed Cell Sorter will overcome all of these limitations and will allow us a) to sort at significantly higher speed, which is critical to sort rare populations and generate more user time due to shortened sorts, b) to sort based on more independent fluorescence parameters, which is critical for the identification of particular immune cell subsets, c) to increase the effective sorting available to all investigators due to a significantly shortened setup period, d) ensure continued service and parts support by the manufacturer, Beckman Coulter. Although the individual experiments and procedures used by the investigators will likely change over time, the open architecture of the requested sorter was designed by the manufacturer to facilitate future modifications as needed. This instrument will be exclusively operated and maintained by the dedicated full-time personnel of the Flow Cytometry Core Facility. Because the research efforts of essentially all investigators are critically dependent on a high speed cell sorter Trudeau Institute is fully committed to provide all the necessary support to house and maintain this instrument in the future. PUBLIC HEALTH RELEVANCE: The cells of the immune system are indispensable for protecting us from invading pathogens and provide long-lasting immunity to re-infection. Many of these cells are rare and can only be identified by a combination of numerous parameters. In order to study the properties and functions of these cells we need to be able to isolate distinct populations based on their unique features.