MicroBrightField Bioscience Imaging System

描述（由申请人提供）：本申请申请资金用于购买Micro Bright Field （MBF）生物科学成像系统，该系统由两个组件组成：Neurolucida （http://www.mbfbioscience.com/neurolucida）和Stereo Investigator （http://www.mbfbioscience.com/stereo-investigator/stereo-investigator）。该系统将为美国国立卫生研究院资助的南伊利诺伊大学医学院（SIU-SOM）斯普林菲尔德的研究人员提供以下能力：1)细胞重建、3D制图和形态测量，旨在量化具有特定特性的细胞亚群的形态特征（Neurolucida）；2)与细胞发育、凋亡、存活、再生、功能和特定蛋白表达相关的立体学研究（Stereo调查员）。这些成像能力对于分析和表征细胞的形态和细胞化学特性，以及细胞亚群的相互联系，以及形态和细胞化学特性与细胞功能和特定蛋白质表达的相关性至关重要。目前，在SIU-SOM没有类似的成像系统，并且没有类似的成像系统提供给邻近大学的SIU-SOM教师。几个正在进行的研究项目，无论是由美国国立卫生研究院资助的，还是预计将在2009年由美国国立卫生研究院资助的，将大大改善了MBF系统在SIU-SOM的可用性。在没有MBF系统的情况下，一些SIU-SOM的研究人员（例如Uteshev博士）使用他们的合作链接访问其他大学（田纳西州孟菲斯的田纳西大学健康科学中心，381英里远，肯塔基州列克星敦的肯塔基大学，396英里远）可用的MBF系统。其他没有进入MBF系统的SIU调查人员(例如，dr。Caspary和Rybak)不得不删减他们的研究计划，以排除需要使用MBF系统的实验。本提案提供了申请NIH基金购买一套MBF系统（Neurolucida和Stereo Investigator）的理由。拟议的购买将极大地有利于SIU- som的基础医学和临床研究项目，将扩大SIU研究人员解决的科学问题的范围，并将提高SIU研究的效率和生产力。参与研究的研究者是药理学、内科和外科部门的教员，并得到NIH各种资金来源的支持（NIDDK， NIDCD， NIA， NINDS）。

DESCRIPTION (provided by applicant): This application requests funds to purchase one Micro Bright Field (MBF) bioscience imaging system composed of two components: Neurolucida (http://www.mbfbioscience.com/neurolucida) and Stereo Investigator (http://www.mbfbioscience.com/stereo-investigator/stereo-investigator). The system will provide NIH-funded investigators at Southern Illinois University School of Medicine (SIU-SOM) Springfield, IL with the ability to conduct: 1) cellular reconstruction, 3D mapping and morphometry aimed at quantifying morphological characteristics of subsets of cells with specific properties (Neurolucida); and 2) stereological studies related to cellular development, apoptosis, survival, regeneration, function and expression of specific proteins (Stereo Investigator). These imaging capabilities are essential for analysis and characterization of morphological and cytochemical properties of cells as well as interconnectivity of subsets of cells and correlation of morphological and cytochemical properties with cellular functions and expression of specific proteins. At present, there is no imaging system with similar capabilities available at SIU-SOM and no similar imaging systems are available to SIU-SOM faculty members at neighboring universities. Several ongoing research projects either funded by the NIH or anticipated to be funded by the NIH in 2009 would be considerably improved by the availability of a MBF system at SIU-SOM. In the absence of MBF system, some SIU-SOM investigators (e.g., Dr. Uteshev) use their collaborative links to access MBF systems available at other universities (University of Tennessee Health Science Center, Memphis, TN; 381 miles away and University of Kentucky, Lexington, KY; 396 miles away). Other SIU investigators who do not have an access to MBF systems (e.g., Drs. Caspary and Rybak) have to truncate their research proposals to exclude experiments requiring usage of a MBF system. This proposal provides justification for the request of NIH funds to purchase one MBF system (Neurolucida and Stereo Investigator). The proposed purchase will greatly benefit basic medical and clinical research programs at SIU-SOM, will broaden the spectrum of scientific questions addressed by SIU investigators, and will increase the efficiency and productivity of SIU research. The participating investigators are faculty members of the Departments of Pharmacology, Internal Medicine and Surgery and are supported by the variety of sources of NIH funding (NIDDK, NIDCD, NIA, NINDS).