BioPlex 200 System with High Throughput Fluidics (HTF) Multiplex Array System

描述（由申请人提供）：本提案是购买Bio-Rad Bio-Plex 200多路阵列系统，具有高通量流体。Bio-Plex将用于印第安纳大学医学院（IUSOM）医学、病理学、儿科和微生物学/免疫学部门的基础和转化研究。NIH资助的8个项目的主要用户可以从Bio-Plex中受益，他们是该提案的一部分，包括：Orschell， Srour和Broxmeyer博士研究造血干细胞生物学，移植和基因治疗。Kapur, Clapp， Turchi和Flockhart，他们研究癌症机制，治疗和DNA修复，Vidal博士专注于神经退行性脑疾病。这些用户目前采用检测细胞因子、趋化因子、核酸、特定蛋白磷酸化、snp和基因表达的方法，这些方法可以很容易地适应使用Bio-Plex进行更有效的多重分析。这些用户将占Bio-Plex使用人数的75%以上，如果这项应用得到资助，将计划招募其他用户。在我们的校园里有一台Luminex 100多路分析仪，但它已经被大量使用，因此无法供我们使用。Bio-Plex 200将被安置在现有的流式细胞术资源设施（FCRF）中，由Srour博士和FCRF优秀的技术人员指导。FCRF是IUSOM内地位良好的核心设施，并且已经制定了许多管理其仪器的科学监督和管理的行政政策。FCRF利用用户退款系统来支付设施的运营费用，因此在财政上是自给自足的。IUSOM完全支持这一建议和FCRF的未来，FCRF即将落户的一个新的集中研究大楼就是明证。预计Bio-Plex不仅将极大地帮助本提案中列出的主要用户的研究工作，而且还将帮助IUSOM中的许多其他科学家，他们将使用Bio-Plex更有效地为当前和未来的拨款申请生成数据。因此，Bio-Plex将在促进IUSOM的使命方面发挥重要作用，即促进癌症和其他疾病的病因、诊断、预防和治疗方面的基础、转化和临床研究，以改善国民的健康和福祉。

DESCRIPTION (provided by applicant): This proposal is for the purchase of a Bio-Rad Bio-Plex 200 Multiplex Array System with high throughput fluidics. The Bio-Plex will be used for basic and translational research in the Departments of Medicine, Pathology, Pediatrics, and Microbiology/Immunology within the Indiana University School of Medicine (IUSOM). Eight major users with NIH funded projects that can benefit from the Bio-Plex are part of this proposal and include: Drs. Orschell, Srour, and Broxmeyer studying hematopoietic stem cell biology, transplantation, and gene therapy, Drs. Kapur, Clapp, Turchi, and Flockhart, who investigate cancer mechanisms, treatments, and DNA repair, and Dr. Vidal who is focused on neurodegenerative brain diseases. These users currently employ methodologies that detect cytokines, chemokines, nucleic acids, specific protein phosphorylation, SNPs, and gene expression that can easily be adapted to more efficient multiplex analyses using the Bio-Plex. These users will comprise more than 75% of the usage of the Bio-Plex, and plans are in place to recruit other users should this application be funded. There is one Luminex 100 multiplex analyzer on our campus, but it is heavily utilized already and thus unavailable for our use. The Bio-Plex 200 will be housed in the existing Flow Cytometry Resource Facility (FCRF) under the direction of Dr. Srour and the excellent technical staff of the FCRF. The FCRF is a core facility in good standing within the IUSOM, and already has many of the administrative policies governing scientific oversight and management of its instruments already in place. The FCRF utilizes a user charge-back system to cover operational costs of the facility, and is thus self-sufficient financially. The IUSOM fully supports this proposal and the future of the FCRF, as evidenced by construction of a new centralized Research building where the FCRF will soon be located. It is expected that the Bio-Plex will greatly aid the research endeavors of not only the major users listed in this proposal, but also many other scientists within the IUSOM who will use the Bio-Plex to more efficiently generate data for current and future grant applications. Thus, the Bio-Plex will play an important role in furthering the mission of the IUSOM to promote basic, translational and clinical research in the causes, diagnosis, prevention, and cure of cancer and other diseases in order to improve health and well being of the Nation.