BD LSR II Special Order System

描述（由申请人提供）：本申请中要求的仪器是Becton Dickinson (BD) LSR II流式细胞仪的配置。BD LSR II的这种配置有五个激光器；（蓝色激光/红色激光/黄绿色激光/紫色激光/紫外激光）和BD高通量取样器（HTS）。已经确定了9个具有高级流式细胞术特定需求的合格用户。所有9个用户都位于两座校外建筑中，虽然它们彼此靠近，但距离校园流式细胞术中心有很大距离，该中心存放的流式细胞仪具有一些必需的技术，但已被过度订购，并且在没有技术支持的正常工作时间之后通常必须访问。为了满足主校区外研究人员对流式细胞术日益增长的需求，校内流式细胞术中心建立了两个卫星设施。作为分子和实验医学（MEM）部门的一部分，卫星流式细胞术设施是唯一一个校外LSR II细胞仪的理想地点，因为有指定的空间，技术支持和大量使用流式细胞术进行研究的研究人员。该卫星设施容纳了两个已有8年历史的FACSCaliburs，其使用的技术无法支持需要超过4种颜色的实验日益增长的需求。此外，他们无法执行用户组多个成员所要求的专门任务。校外研究人员获得BD LSR II将提供急需的仪器，并极大地促进NIH资助的流感、哮喘、败血症、自身免疫、艾滋病毒/艾滋病、癌症、衰老、干细胞、大分子成像、细胞骨架和细胞形状以及蛋白质-碳水化合物相互作用等领域的研究。

DESCRIPTION (provided by applicant): The instrument requested in this application is a configuration of the Becton Dickinson (BD) LSR II Flow Cytometer. This configuration of the BD LSR II has five lasers; (Blue laser/Red laser/Yellow-Green laser/Violet laser/UV laser) and the BD High Throughput Sampler (HTS). Nine qualified users have been identified with specific needs for advanced flow cytometry. All nine users are situated in two off-campus buildings which while close to each other are a significant distance from the on-campus Flow Cytometry Core which houses flow cytometers that have some of the required technology but are oversubscribed and often have to be accessed after normal working hours when technical support is not available. To service the growing need for analytical flow cytometry by investigators housed outside the main campus the on-campus Flow Cytometry Core has established two satellite facilities. The satellite flow facility that is part of the Department of Molecular and Experimental Medicine (MEM) is ideally situated as the site for the only off-campus LSR II cytometer due to the presence of designated space, technical support and a significant population of investigators who use flow cytometry in their research. This satellite facility houses two FACSCaliburs that are eight years old and use technology that is unable to support the growing need for experiments requiring greater than 4 colors. In addition they are incapable of performing the specialized tasks required by multiple members of the User Group. Access to a BD LSR II by off-campus investigators would provide much needed instrumentation and greatly facilitate NIH funded studies in the areas of influenza, asthma, sepsis, autoimmunity, HIV/AIDS, cancer, aging, stem cells, macromolecular imaging, cytoskeleton and cell shape, and protein-carbohydrate interactions. PUBLIC HEALTH RELEVANCE: The Becton Dickinson LSR II is a versatile and powerful flow cytometer capable of multi-color, multiparameter analysis. Such an instrument is an invaluable tool in identifying the numerous cell populations and their subsets that contribute to many diseases. The availability of such an instrument to investigators in the Department of Molecular and Experimental Medicine of the Scripps Research Institute will greatly facilitate cutting-edge research in the areas of influenza, asthma, sepsis, autoimmunity, HIV/AIDS,cancer, aging, stem cells, macromolecular imaging, cytoskeleton and cell shape, and proteincarbohydrate interactions.