Biofilm composition and secondary caries in situ and in vivo

描述（由申请人提供）：继发性或复发性龋齿被认为是在修复体附近发展的龋齿病变。龋病的基本病因被认为存在于菌斑生物膜内。由于频繁摄入可发酵的碳水化合物，该生物膜从与牙齿组织平衡的状态改变为促进脱矿质的状态。虽然复发性龋的基本病因与原发性龋没有什么不同，但有几个因素使复发性龋变得特殊。在该过程的位点处的非生物材料的存在可以以几种方式影响该过程：1）材料和牙齿之间的界面/间隙可以产生新的生物膜位点和/或改变扩散行为; 2）材料性质，例如缺乏缓冲能力，可以影响生物膜和/或化学过程的发展和组成。在这个项目中，我们专注于生物膜与复发性龋齿病变的发展，在体内和原位。关于生物膜，我们最感兴趣的特征是其组成和产酸。将使用传统的培养方法和最现代的基于DNA的分子微生物学技术：焦磷酸测序来研究生物膜的组成。体内研究将使我们能够了解最常见的修复材料复合材料和汞合金的原发性和继发性病变生物膜之间的差异。两项原位研究将使我们能够评估修复材料特性对生物膜组成和功能以及病变发展的影响。此外，复合材料修复体和牙齿之间的间隙的作用可以评估，再次相对于生物膜组成和功能以及病变发展。具有抗菌和/或抗龋齿成分的材料将被纳入研究。通过这种方式，我们希望阐明材料性能和修复界面条件对复发性龋齿的作用，希望为改善复合材料修复性能指明方向。 公共卫生相关性：生物膜在龋病病因学中的重要性已得到公认。最近，生物膜的生态和功能的作用的观点已经发展和现代分子技术，如焦磷酸测序，将使我们能够阐明这一作用，特别是在继发性龋齿领域。我们的研究将使人们能够更好地了解继发龋的发展过程，这是一个先决条件，为改善复合材料和修复技术，以提高其使用寿命。

DESCRIPTION (provided by applicant): Secondary or recurrent caries is considered to be a caries lesion developing adjacent to a restoration. The basic etiology of caries is recognized to lie within the plaque biofilm. This biofilm, due to the frequent ingestion of fermentable carbohydrates, changes from a state of equilibrium with the tooth tissue to a demineralization promoting state. Although the basic etiology of recurrent caries is no different from that of primary caries, there are several factors that make recurrent caries special. The presence of a non-biological material at the site of the process may influence this process in several ways: 1) the interface / gap between the material and the tooth may create a new biofilm site and/or change the diffusion behavior; 2) the material properties, such as a lack in buffering capacity, may influence the development and composition of the biofilm and/or the chemical process. In this project we focus on the biofilm in relation to recurrent caries lesion development both in vivo and in situ. The characteristics we are most interested in regarding the biofilm are its composition and acid production. Biofilm composition will be studied both using traditional culturing methods, and using the most modern DNA-based molecular microbiology technique: pyrosequencing. The in vivo study will allow us to look at differences between primary and secondary lesion biofilm, for the most common restorative materials composite and amalgam. Two in situ studies will allow us to evaluate the effect of restorative material properties on biofilm composition and function and lesion development. Also, the role of gaps between composite restoration and tooth can be evaluated, again with respect to both biofilm composition and function and lesion development. Materials with anti-bacterial and/or anti- caries components will be included in the studies. In this way we hope to elucidate the role of material properties and restoration interface conditions on recurrent caries, hopefully pointing the way towards improving composite restoration performance. PUBLIC HEALTH RELEVANCE: The importance of the biofilm in caries etiology is well recognized. Recently, views on the role of biofilm ecology and function have evolved and modern molecular techniques, such as pyrosequencing, will enable us to shed light on this role especially in the field of secondary caries. Our study will make it possible to better understand the process of secondary caries development, which is a prerequisite for improvement of composite materials and restoration techniques in order to enhance their service lifetime.