Neurochemical and Behavioral Correlates of ETOH Effects

描述(由申请人提供)：这是一项继续提供培训补助金的建议，以支持7名博士后和2名博士后研究员，他们将接受广泛和深入的培训，进行酒精中毒研究。培训领域涵盖最先进的方法，包括分子生物学和遗传学、电生理学、细胞成像、神经化学和行为学。研究模型包括动物和人类。该培训计划将促进和支持合作研究。分子生物学培训将包括研究细胞表达系统中配体门控离子通道的功能(Mihic，Harris)，开发新的转基因动物模型(Atkinson，Harris，Mihic)，以及识别乙醇反应基因(Iyer，Bergeson，Harris，Mihic，Atkinson)。遗传学培训将包括对人类和动物受试者(冈萨雷斯、阿特金森、弗罗姆、哈里斯、伯格森、伊耶)进行基因分型。电生理和显微成像训练将包括在单个细胞和脑片(Morikawa、Morrisett、Mihic、Harris)中使用全细胞和细胞内方法进行实验。神经化学的培训将侧重于细胞内和细胞外的信号机制，重点是磷酸化(Morrisett)，谷氨酸、多巴胺和其他神经递质的释放和运输(Gonzales，Harris，Mayfield)，以及突触蛋白的表达(Mayfield，Morikawa，Morrisett)。将进行酒精对行为影响(运动技能、强化、焦虑和戒断)的研究培训(Duvuchelle，Harris，Gonzales，Bergeson，Schallert)。还将进行酒精研究的心理社会方面的培训(Fromme)以及人类中的基因型和饮酒的相互作用(Fromme、Bergeson、Harris、Iyer)。博士后研究员将在培训开始时选择一个项目。博士前研究生培训将轮流在选定的教职员工的实验室中进行，然后再为论文研究选择导师。博士前学生将被要求完成一系列核心课程要求，涵盖乙醇对中枢神经系统的作用、科学伦理、实验设计和统计分析。我们将继续以少数族裔招聘为重点。该培训项目有着出色的记录，并将继续专注于培养合格的酒精研究人员。

DESCRIPTION (provided by applicant): This is a proposal for the continuation of a training grant to support seven predoctoral and two postdoctoral fellows who will be broadly and intensively trained to conduct research on alcoholism. Training areas span the breadth of state-of-the-art approaches including molecular biology and genetics, electrophysiology, cellular imaging, neurochemistry, and behavior. Research models include both animal and human. The training program will promote and support collaborative research. Molecular biology training will encompass studies of the function of ligand-gated ion channels in cellular expression systems (Mihic, Harris), development of new transgenic animal models (Atkinson, Harris, Mihic), and identification of ethanol responsive genes (Iyer, Bergeson, Harris, Mihic, Atkinson). Genetics training will involve genotyping of human and animal subjects (Gonzales, Atkinson, Fromme, Harris, Bergeson, Iyer). Electrophysiological and microscopic imaging training will comprise experiments with whole cell and intracellular methods in single cells and in brain slices (Morikawa, Morrisett, Mihic, Harris). Training in neurochemistry will focus on intracellular and extracellular signaling mechanisms with an emphasis on phosphorylation (Morrisett), release and transport of glutamate, dopamine, and other neurotransmitters (Gonzales, Harris, Mayfield), and expression of synaptic proteins (Mayfield, Morikawa, Morrisett). Research training in the behavioral effects of ethanol (motor skills, reinforcement, anxiety, and withdrawal) will be done (Duvauchelle, Harris, Gonzales, Bergeson, Schallert). Training in psychosocial aspects of alcohol research (Fromme) and the interaction of genotype and alcohol drinking in humans (Fromme, Bergeson, Harris, Iyer) will also be done. Postdoctoral fellows will choose a project at the onset of their training. Predoctoral graduate training will rotate through the laboratories of selected faculty members prior to choosing an advisor for dissertation research. Predoctoral students will be required to complete a series of core course requirements covering ethanol's actions on the central nervous system, scientific ethics, experimental design, and statistical analysis. We will continue to focus on minority recruitment. The training program has an excellent record and will continue to focus on producing well qualified alcohol researchers.