Cells and Tissue Core

这个核心的目标是分离和繁殖原代人类白细胞(单核细胞和 外周血淋巴细胞，并维持病理组织的脑库[用于所有项目和核心]。 此外，该核心还将提供小鼠骨髓来源的巨噬细胞、人和小鼠神经元 和神经胶质细胞，用于研究神经前体细胞和神经艾滋病的基于细胞的纳米制剂。严谨 对于这个整合得很好的核心，质量控制措施已经到位。这些设施的实质性用途是 在上一个供资周期内向所有调查人员提供的资料。原生人类白细胞将被分离 用离心法将HIV-1、HIV-2和乙肝、丙型肝炎血清阴性献血者的单核细胞和淋巴细胞 淘洗。人类胶质细胞(小胶质细胞和星形胶质细胞)和/或神经元将从胎儿组织中获得。这个 将从人类和小鼠脑组织中提纯、鉴定和鉴定神经元、胶质细胞和/或祖细胞。 提供实验(所有项目)。智库也将通过这一核心运作。 有236个样本可用，其中包括神经病理诊断为阿尔茨海默病、艾滋病毒-1相关性痴呆和其他神经退行性疾病(包括但不限于中风、帕金森病和多发性硬化症)的组织。死于与神经无关的疾病的患者 系统充当年龄匹配的对照。这些来自脑库的成人原代细胞和其他组织 将在内部用于制备RNA和蛋白质样本库。脑组织裂解物中的RNA和蛋白质 调查和冰冻和石蜡组织的免疫组织化学将是一个可用的资源。这些 将提供常见疾病终点的数据。总之，这个‘细胞和组织核心’将提供所有 解决项目研究目标所需的生物标本并利用 通过这个基础设施获得的仔细控制的样本，以研究神经免疫和它的 与神经退行性变有关。

The objectives of this core are to isolate and propagate primary human leukocytes (monocytes and peripheral blood lymphocytes and maintain a brain bank of pathological tissues [for all projects and cores]. In addition, the core will provide murine bone-marrow-derived macrophages, human and murine neuronal and glial cells for studies of neural progenitors and cell-based nanoformulations in neuroAIDS. Rigorous quality control measures are in place for this well-integrated core. Substantive uses of these facilities were provided to all investigators during the previous funding cycle. Primary human leukocytes will be fractionated into monocytes and lymphocytes from HIV-1, 2 and hepatitis B and C seronegative donors by centrifugal elutriation. Human glia (microglia and astrbcytes) and/or neurons will be obtained from fetal tissues. The neurons, glia, and/or progenitor cells from human and mouse brain tissue will be purified, characterized and provided for experiments (all projects). The Brain Bank will also be operated through this core. Two hundred and thirty-six specimens are available, which include tissues with neuropathological diagnoses of Alzheimer's disease, HIV-1-associated dementia and other neurodegenerative disorders (including but not limited to stroke, Parkinson's disease, and multiple sclerosis). Patients who died of diseases not related to the nervous system serve as age-matched controls. These primary adult human cells and other tissues from Brain Bank will be utilized within to prepare RNA and protein specimen banks. Brain tissue lysates for RNA and protein investigations and frozen and paraffin tissues for immunohistochemistry will be an available resource. These will provide data for common endpoints of disease. In toto, this 'cells and tissue core' will provide all the biological specimens necessary to address research objectives of the program project and utilize the carefully controlled specimens obtained through this infrastructure to investigate neural immunity and its links to neurodegeneration.