Magneto-Nano Diagnostic and Analytical Devices for Cancer

项目概述：研究项目二以设计和翻译研究为中心 应用新型可调磁性纳米颗粒(MNP)、超灵敏磁性传感器芯片以及用于癌症生物标记物和细胞的高效磁性分选器。如图N3.2.1所示，产生的磁纳米蛋白质芯片平台将检测低至全摩尔浓度的蛋白质生物标志物(Aim 1.A)，并将用于监测肺癌的治疗反应(Aim 1.B)和卵巢癌的早期诊断(Aim 1.0)。产生的磁性筛子(Aim 2.A)和可调MNP(Aim 2.B)将用于从肺癌患者或卵巢癌干细胞中采集循环肿瘤细胞，捕获和释放效率前所未有的&gt；90%(Aim 2.C)，对癌症诊断和治疗都至关重要。这些稀有细胞将利用AIM 1以及项目3(N3.3.5.2.1.3)中的纳米技术，通过单细胞蛋白质和核酸图谱以及分泌组分析进行讯问，以进一步阐明癌症途径和转移(AIM 3)。将纳米技术中的这些进展应用于临床前模型和临床对象将导致对癌症的更好理解和治疗。

Project Overview: Research Project 2 is centered on the translational research of devising and applying novel and tunable magnetic nanoparticles (MNP), ultrasensitive magnetic sensor chips, and highly efficient magnetic sorters for cancer biomarkers and cells. As illustrated in Figure N3.2.1, the resulting magneto-nano protein chip platform will detect protein biomarkers down to attomolar concentrations (Aim 1.A) and will be used for monitoring therapeutic response of lung cancer (Aim 1.B) and early diagnosis of ovarian cancer (Aim 1.0). The resulting magnetic sifter (Aim 2.A) and tunable MNP (Aim 2.B) will be used to harvest circulating tumor cells from lung cancer patients or ovarian cancer stem cells with unprecedented capture and release efficiencies of >90% (Aim 2.C), critical for both cancer diagnosis and treatment. These rare cells will be interrogated with the nanotechnologies in Aim 1, as well as Project 3 (N3.3.5.2.1.3), by single cell protein and nucleic acid profiling and secretome analysis to further elucidate cancer pathways and metastasis (Aim 3). The application of these advances in nanotechnology to pre-clinical models and clinical subjects will lead to better understanding and treatment of cancer.