Proteomic-based Approach to Ventilator Associated Pneumonia

描述(由申请人提供)：这项拟议的职业生涯中期研究员奖的目标是通过加强指导和扩大以患者为导向的研究计划，在肺和危重病科内扩大以患者为导向的翻译研究培训。这项提议将为施纳普博士提供足够的资源，以继续建立她的以患者为中心的研究计划(检查肺损伤和感染的界面)，并确保有足够的保护时间，通过将初级教员和研究员纳入该计划，为他们提供更多指导机会。此外，它还将为Schnapp博士参加额外的课程工作提供必要的保护时间，以进一步促进她作为一名以患者为中心的研究人员的发展。该研究计划的目标是分析呼吸机相关肺炎(VAP)期间的空气环境，以期深入了解VAP的致病机制，洞察ARDS背景下宿主对感染的反应，并提高对高危患者VAP的诊断。这一信息可能会根据已确定的途径建议新的治疗方式，或者建议VAP的其他确认性诊断标准。该项目建立在目前资助的项目基础上，包括NIH急性肺损伤SCCOR项目和新资助的RO1项目，以开发蛋白质组方法来分析肺损伤期间的支气管肺泡灌洗液。该指导计划扩大了施纳普在指导年轻医生进行以患者为导向的研究方面的非常积极的作用，并扩大了她在为学员制定翻译研究课程方面的领导作用。研究和指导计划将利用华盛顿大学的杰出资源和基础设施，包括肺科与公共卫生学院的长期互动，现有的K30和K12计划，以及最先进的蛋白质组设施。华盛顿大学为肺部医学方面的协作培训和以患者为中心的研究提供了一个出色的环境。

DESCRIPTION (provided by applicant): The goal of this proposed midcareer investigator award is to expand training in patient-oriented translational research within the Pulmonary and Critical Division through enhanced mentoring and expansion of patient-oriented research program. This proposal will provide sufficient resources for Dr. Schnapp to continue to build her patient-oriented research program (examining the interface of lung injury and infection) and to insure sufficient protected time to provide more opportunities for mentoring junior faculty and fellows by integrating them into this program. It addition, it will provide the necessary protected time for Dr Schnapp's participation in additional coursework to further enhance her own development as a patient-oriented researcher. The goal of the research plan is to analyze the airspace environment during ventilator associated pneumonia (VAP) in hopes of obtaining insight into the pathogenic mechanisms of VAP, provide insight into the host response to infection in the setting of ARDS and improve the diagnosis of VAP in high-risk patients. This information may suggest new therapeutic modalities based on identified pathways, or suggest additional confirmatory diagnostic criteria for VAP. This program builds on currently funded programs, including NIH SCCOR program in Acute Lung injury, and newly funded RO1 to develop proteomic methodologies to analyze the bronchoalveolar lavage fluid during lung injury. The mentoring plan expands Dr. Schnapp's very active role in mentoring of young physicians in patient-oriented research and her leadership role in developing a curriculum in translational research for trainees. The research and mentoring program will take advantage of outstanding resources and infrastructure at the University of Washington, including long standing interactions of the Pulmonary Division with the School of Public Health, existing K30 and K12 programs, and state-of- the-art proteomic facilities. The University of Washington provides an outstanding setting for collaborative training and patient-oriented research in Pulmonary Medicine.